Leifa F, Pandey A, Soccol C R
Departamento de Engenharia Quimica, Universidade Federal do Parana, Curitiba-PR, Brazil.
J Basic Microbiol. 2000;40(3):187-97. doi: 10.1002/1521-4028(200007)40:3<187::AID-JOBM187>3.0.CO;2-Q.
Studies were carried out to screen twelve strains of Lentinus edodes for their efficiency to grow on toxic agro-industrial residues of coffee industry in solid state cultivation (SSC). Based on best mycelial growth (7.57 mm/day) and biomass production (48.78 mg/plate in 12 days at 24 degrees C) in coffee husk extract medium, a strain, L. edodes LPB 02 was selected for mushroom cultivation in SSC on coffee husk (treated and untreated), coffee spent ground, and a mixed-substrate comprising coffee husk and coffee spent ground (1:1). SSC was carried out under different conditions of moisture and spawn rate. Spawn rate of 10% and moisture level of 55-60% was found suitable for all the substrates. Treatment of the coffee husk with hot water was found useful for its utilization by the fungus. Results showed that there was an increase in the protein content and decrease in the fibre content of the substrates after SSC. Fruiting bodies were obtained from the treated coffee husk, spent ground and mixed-substrate, and the biological efficiency achieved was 85.8, 88.6 and 78.4% for these substrates, respectively. However, no fruiting body was obtained with raw coffee husk was used as the substrate. Results showed that after SSC, there was a decrease of about 27, 40 and 24% in caffeine and about 18, 49 and 12% in tannin contents in the treated coffee husk, coffee spent ground and mixed substrate, respectively. No caffeine or, tannins were found in fruiting body indicating their degradation by the fungal strain.
开展了多项研究，以筛选12种香菇菌株在固态培养（SSC）中利用咖啡行业有毒农业工业残渣生长的效率。基于在咖啡壳提取物培养基中最佳的菌丝体生长（7.57毫米/天）和生物量产量（24摄氏度下12天内48.78毫克/平板），选择了一株香菇LPB 02菌株，用于在SSC中以咖啡壳（处理过的和未处理的）、咖啡渣以及由咖啡壳和咖啡渣（1:1）组成的混合基质进行蘑菇栽培。SSC在不同的水分和接种率条件下进行。发现10%的接种率和55 - 60%的水分水平适用于所有基质。发现用热水处理咖啡壳有利于真菌对其的利用。结果表明，SSC后基质的蛋白质含量增加，纤维含量降低。从处理过的咖啡壳、咖啡渣和混合基质中获得了子实体，这些基质的生物学效率分别达到85.8%、88.6%和78.4%。然而，以生咖啡壳为基质未获得子实体。结果表明，SSC后，处理过的咖啡壳、咖啡渣和混合基质中的咖啡因含量分别下降了约27%、40%和24%，单宁含量分别下降了约18%、49%和12%。在子实体中未发现咖啡因或单宁，表明它们被该真菌菌株降解。
