Brand D, Pandey A, Roussos S, Soccol CR
Laboratorio de Processos Biotecnologicos, Departamento Engenharia Quimica, Universidade Federal do Parana (UFPR), 81531-970, Curitiba-PR, Brazil
Enzyme Microb Technol. 2000 Jul 1;27(1-2):127-133. doi: 10.1016/s0141-0229(00)00186-1.
Studies were carried out on detoxification of coffee husk in solid state fermentation using three different strains of Rhizopus, Phanerochaete, and Aspergillus sp. Fungal strains were selected by their ability to grow on a coffee husk extract-agar medium. Using R. arrizus LPB-79, the best results on the degradation of caffeine (87%) and tannins (65%) were obtained with pH 6.0 and moisture 60% in 6 days. When P. chrysosporium BK was used, maximum degradation of caffeine and tannins were 70.8 and 45%, respectively, with coffee husk having 65% moisture and pH 5.5 in 14 days. The Aspergillus strain, isolated from the coffee husk, showed best biomass formation on coffee husk extract-agar medium. Optimization assays were conducted using factorial design, and surface response experiments with Aspergillus sp. The best detoxification rates achieved were 92% for caffeine and 65% for tannins. The results showed good prospects of using these fungal strains, in particular Aspergillus sp., for the detoxification of coffee husk.
利用三种不同的根霉、黄孢原毛平革菌和曲霉属菌株对咖啡壳进行固态发酵脱毒研究。根据真菌菌株在咖啡壳提取物琼脂培养基上的生长能力来选择菌株。使用米根霉LPB - 79，在pH值为6.0、湿度为60%的条件下，6天内咖啡因降解率（87%）和单宁降解率（65%）达到最佳效果。当使用黄孢原毛平革菌BK时，在湿度为65%、pH值为5.5的咖啡壳中，14天内咖啡因和单宁的最大降解率分别为70.8%和45%。从咖啡壳中分离出的曲霉属菌株在咖啡壳提取物琼脂培养基上表现出最佳的生物量形成。使用析因设计进行优化试验，并对曲霉属菌株进行表面响应实验。获得的最佳脱毒率为咖啡因92%，单宁65%。结果表明，利用这些真菌菌株，特别是曲霉属菌株对咖啡壳进行脱毒具有良好的前景。
