Bachtrog D, Agis M, Imhof M, Schlötterer C
Institut für Tierzucht und Genetik, Vienna, Austria.
Mol Biol Evol. 2000 Sep;17(9):1277-85. doi: 10.1093/oxfordjournals.molbev.a026411.
Recently, the use of microsatellites as genetic markers has become very popular. While their evolutionary dynamics are not yet fully understood, the emerging picture is that several factors are influencing microsatellite mutation rates. Recent experiments demonstrated a significant effect of repeat motif length on microsatellite mutation rates. Here, we studied the influence of the base composition of the microsatellite. Forty-two microsatellite loci on the second chromosome with the three most abundant dinucleotide repeat motifs (TC/AG, AT/TA, GT/CA) were characterized for six different Drosophila melanogaster populations. Applying ANOVA to the variance in repeat number, we found a significant influence of repeat motif on microsatellite variability. Calculating relative mutation rates, GT/CA appears to have the highest mutation rate, and AT/TA appears to have the lowest. Similar differences in mutation rates were obtained by an alternative method which estimates microsatellite mutation rates from their genomic length distribution.
最近，微卫星作为遗传标记的应用变得非常普遍。虽然它们的进化动态尚未完全了解，但新出现的情况是，有几个因素正在影响微卫星突变率。最近的实验证明了重复基序长度对微卫星突变率有显著影响。在这里，我们研究了微卫星碱基组成的影响。对六个不同的黑腹果蝇种群的第二条染色体上具有三种最丰富的二核苷酸重复基序（TC/AG、AT/TA、GT/CA）的42个微卫星位点进行了表征。对重复数目的方差应用方差分析，我们发现重复基序对微卫星变异性有显著影响。计算相对突变率，GT/CA似乎具有最高的突变率，而AT/TA似乎具有最低的突变率。通过另一种从基因组长度分布估计微卫星突变率的方法也获得了类似的突变率差异。
