Kolmert A, Wikström P, Hallberg K B
School of Biological Sciences, University of Wales, Gwynedd LL57 2UW, Bangor, UK.
J Microbiol Methods. 2000 Aug;41(3):179-84. doi: 10.1016/s0167-7012(00)00154-8.
A standard turbidimetric assay for the determination of sulfate in water was modified with the objective of achieving a quick and simple method for monitoring the decrease of sulfate in cultures of sulfate-reducing bacteria. The effects of sulfate concentration, mixing time and the ratio of sample to conditioning reagent were optimized using a central composite face-centered response surface model design. The results suggested that a mixing time of 30 s resulted in smaller absorbance variance, the variance in absorbance measurements tended to increase with concentration of sulfate and that the ratio between the amount of conditioning reagent and sample had no significant influence on the absorbance variance. The modified assay thus developed is simple and quick, and covers a comparatively large sulfate concentration range (0-5 mM) compared to the standard turbidimetric assay.
为了实现一种快速简便的方法来监测硫酸盐还原菌培养物中硫酸盐的减少，对用于测定水中硫酸盐的标准比浊法进行了改进。使用中心复合表面响应模型设计优化了硫酸盐浓度、混合时间以及样品与调节试剂比例的影响。结果表明，30秒的混合时间导致吸光度方差较小，吸光度测量的方差倾向于随着硫酸盐浓度的增加而增大，并且调节试剂与样品量之间的比例对吸光度方差没有显著影响。由此开发的改进方法简单快速，与标准比浊法相比，其覆盖的硫酸盐浓度范围相对较大（0-5 mM）。
