Wu D C, Yu Y P, Lee N T, Yu A C, Wang J H, Han Y F
Department of Biochemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, China.
Neurochem Res. 2000 Jul;25(7):923-9. doi: 10.1023/a:1007544106645.
The expression of cyclin-dependent kinase 5 (Cdk5) and its regulatory subunits, p35 and p39, was investigated in rat brain from embryonic day 12 (E12) to postnatal 18 months (18M). The Cdk5 protein levels increased from E12 to postnatal day 7 (P7) and remained at this level until 18M. The Cdk5 kinase activity and the levels of both p35 mRNA and protein were low at E12, became prominent at E18-P14 but then decreased in the adult and aged rat brains of 3M to 18M. In comparison, the expression pattern of p39 appeared to have an inverse relationship to that of Cdk5 and p35. In regional distribution studies, p35 protein levels and Cdk5 kinase activity were significantly higher in the cerebral cortex and hippocampus, but lower in the cerebellum and striatum. These results suggested that Cdk5, p35 and p39 might have region-specific and developmental stage-specific functions in rat brain.
研究了从胚胎第12天（E12）到出生后18个月（18M）大鼠脑中细胞周期蛋白依赖性激酶5（Cdk5）及其调节亚基p35和p39的表达情况。Cdk5蛋白水平从E12到出生后第7天（P7）升高，并在此水平维持至18M。Cdk5激酶活性以及p35 mRNA和蛋白水平在E12时较低，在E18 - P14时显著升高，但在3M至18M的成年和老年大鼠脑中随后下降。相比之下，p39的表达模式似乎与Cdk5和p35的表达模式呈负相关。在区域分布研究中，大脑皮质和海马体中的p35蛋白水平和Cdk5激酶活性显著较高，而在小脑和纹状体中较低。这些结果表明，Cdk5、p35和p39在大鼠脑中可能具有区域特异性和发育阶段特异性功能。
