Nakagawa H, Yamamoto D, Kiyozuka Y, Tsuta K, Uemura Y, Hioki K, Tsutsui Y, Tsubura A
Department of Surgery II, Kansai Medical University, Moriguchi, Osaka, Japan.
J Cancer Res Clin Oncol. 2000 Aug;126(8):448-54. doi: 10.1007/PL00021280.
Genistein, a prominent isoflavone in soy products, produced dose- and time-dependent in vitro growth inhibition at high concentrations (at least 185 microM) with an IC50 of 7.0-274.2 microM after 72 h incubation in four breast cancer cell lines (DD-762, Sm-MT, MCF-7 and MDA-MB-231) and one breast epithelial cell line (HBL- 100) of human and animal origin; it stimulated estrogen-receptor-positive MCF-7 cells at low concentrations (3.7 nM-37 microM). Genistein-exposed cells underwent apoptosis, confirmed by G2/M arrest followed by the appearance of a sub-G1 fraction in cell-cycle progression, and by a characteristic cell ultrastructure. The apoptosis cascade was due to up-regulation of Bax protein, down-regulation of Bcl-XL protein, and activation of caspase-3. Genistein acted in synergism with eicosapentaenoic acid (EPA), a fish oil component, on human breast cancer MCF-7 cells (genistein > 93.2 microM and EPA > 210.9 microM) and on MDA-MB-231 cells (genistein > 176.1 microM and EPA > 609.3 microM). Dietary intake of genistein in combination with EPA may be beneficial for breast cancer control.
染料木黄酮是大豆制品中的一种重要异黄酮，在四种人源和动物源乳腺癌细胞系（DD - 762、Sm - MT、MCF - 7和MDA - MB - 231）以及一种乳腺上皮细胞系（HBL - 100）中，经72小时孵育后，高浓度（至少185微摩尔）时可产生剂量和时间依赖性的体外生长抑制作用，IC50为7.0 - 274.2微摩尔；低浓度（3.7纳摩尔 - 37微摩尔）时可刺激雌激素受体阳性的MCF - 7细胞。暴露于染料木黄酮的细胞发生凋亡，这通过细胞周期进程中G2/M期阻滞随后出现亚G1期部分得以证实，并且通过特征性的细胞超微结构得以证实。凋亡级联反应是由于Bax蛋白上调、Bcl - XL蛋白下调以及caspase - 3激活所致。染料木黄酮与鱼油成分二十碳五烯酸（EPA）协同作用于人乳腺癌MCF - 7细胞（染料木黄酮> 93.2微摩尔且EPA > 210.9微摩尔）以及MDA - MB - 231细胞（染料木黄酮> 176.1微摩尔且EPA > 609.3微摩尔）。饮食中摄入染料木黄酮与EPA相结合可能对乳腺癌控制有益。
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