使用抗牛脑微管蛋白单特异性抗体对有丝分裂纺锤体进行免疫荧光检测。
Immunofluorescence of mitotic spindles by using monospecific antibody against bovine brain tubulin.
作者信息
Fuller G M, Brinkley B R, Boughter J M
出版信息
Science. 1975 Mar 14;187(4180):948-50. doi: 10.1126/science.1096300.
PMID:1096300
Abstract
Monospecific antibody directed against bovine brain tubulin has been purified by affinity chromatography and tested against soluble tubulin and intact microtubules of brain and mitotic apparatus. Binding of the tubulin antibody to the mitotic spindle of rat kangaroo cells was demonstrated in all stages of mitosis by indirect immunofluorescence.
摘要
一种针对牛脑微管蛋白的单特异性抗体已通过亲和层析法纯化，并针对脑和有丝分裂装置的可溶性微管蛋白及完整微管进行了检测。通过间接免疫荧光法在有丝分裂的各个阶段都证实了微管蛋白抗体与大鼠袋鼠细胞有丝分裂纺锤体的结合。
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