Ikeda Y, Fujii Y
Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan.
Biol Pharm Bull. 2000 Aug;23(8):906-10. doi: 10.1248/bpb.23.906.
The preparation and antigenic properties of digoxin C-3' and C-3" hemisuccinate-bovine serum albumin (BSA) conjugates are described. The antisera were prepared by immunizing rabbits with each of the digoxin-BSA conjugates, and properties of the antisera were characterized by RIA with 3H-labeled digoxin. The antidigoxin antiserum from immunization with digoxin 3'-hemisuccinate-BSA conjugate possessed high specificity for digoxin, exhibiting fairly low cross-reactions with dihydrodigoxin (2.1%), digoxigenin monodigitoxoside (0.9%), digoxigenin bisdigitoxoside (0.6%), and digoxigenin (0.1%).
描述了地高辛C - 3'和C - 3''半琥珀酸 - 牛血清白蛋白（BSA）偶联物的制备及其抗原特性。通过用每种地高辛 - BSA偶联物免疫兔子制备抗血清，并用³H标记的地高辛通过放射免疫分析（RIA）表征抗血清的特性。用地高辛3'-半琥珀酸 - BSA偶联物免疫产生的抗地高辛抗血清对地高辛具有高特异性，与二氢地高辛（2.1%）、洋地黄毒苷元单洋地黄毒糖苷（0.9%）、洋地黄毒苷元双洋地黄毒糖苷（0.6%）和洋地黄毒苷元（0.1%）的交叉反应相当低。
