Fujii Y, Teranishi M, Ikeda Y, Yamazaki M, Kishida S, Miyabo S
Steroids. 1985 Oct-Nov;46(4-5):857-65. doi: 10.1016/0039-128x(85)90034-0.
For radioimmunoassay of the catechol estrogens, four hapten-bovine serum albumin (BSA) conjugates were prepared from 6-oxo-2-hydroxyestradiol 6-(O-carboxymethyl)oxime, 2-hydroxyestradiol 17-hemisuccinate, 6-oxo-4-hydroxyestradiol 6-(O-carboxymethyl)oxime and 4-hydroxyestradiol 17-hemisuccinate by coupling with BSA, employing the mixed anhydride method. The antisera elicited in rabbits by immunization with these antigens showed high affinity and specificity for 2-hydroxyestradiol or 4-hydroxyestradiol with cross-reactivities to a few structurally related estrogens. The specificity of antisera obtained is discussed in relation to the site of attachment of the hapten to BSA.
为了进行儿茶酚雌激素的放射免疫测定，通过混合酸酐法将6-氧代-2-羟基雌二醇6-(O-羧甲基)肟、2-羟基雌二醇17-半琥珀酸酯、6-氧代-4-羟基雌二醇6-(O-羧甲基)肟和4-羟基雌二醇17-半琥珀酸酯与牛血清白蛋白(BSA)偶联，制备了四种半抗原-BSA缀合物。用这些抗原免疫家兔产生的抗血清对2-羟基雌二醇或4-羟基雌二醇具有高亲和力和特异性，对一些结构相关的雌激素具有交叉反应性。结合半抗原与BSA的连接位点，讨论了所获得抗血清的特异性。
