Osaki M, Sakatani T, Okamoto E, Goto E, Adachi H, Ito H
First Department of Pathology, Tottori University, Faculty of Medicine, Yonago, Japan.
Virchows Arch. 2000 Jul;437(1):31-6. doi: 10.1007/s004280000205.
Thymidine phosphorylase (dThdPase)/platelet-derived endothelial cell growth factor (PD-ECGF) is expressed at higher levels in a variety of human carcinomas than in adjacent normal tissue. The higher expression is associated with an increase in intratumoral microvessel density (IMVD) and an unfavorable patient prognosis. This study examined the role of dThdPase in apoptosis, IMVD and p53 expression in human gastric carcinomas, dThdPase expression was noted in 12 (35.3%) of 34 early carcinomas, and in 20 (55.6%) of 36 advanced carcinomas. At least 10 areas consisting of carcinoma cells with diffuse dThdPase expression from the 32 dThdPase-positive tumors (category I), and 10 areas without dThdPase expression from the 38 negative tumors (category II) were selected from each case. For early gastric carcinoma, the mean IMVD was 88.8+/-19.4 in category I and 61.4+/-17.3 in category II carcinomas, while for advanced gastric carcinoma, the mean IMVD was 98.8+/-21.0 in category I and 76.0+/-27.1 in category II carcinomas. The mean IMVD was significantly higher in category I than in category II tumors (P<0.05). The mean apoptotic index (AI: percentage of apoptotic cells) was 1.95+/-1.30 in category I, and 3.76+/-1.49 in category II carcinomas for early gastric carcinoma, and 1.51+/-0.98 in category I and 2.14+/-0.66 in category II carcinomas for advanced gastric carcinoma, the value of the mean AI being significantly (P<0.05) higher in dThdPase-negative tumors (category II) than in the positive tumors (category I), regardless of tumor stage or histological type. There was a significant inverse correlation (P<0.001) between AI and IMVD. These results indicate that dThdPase expression is associated with both an increase in intratumoral microvessels and a decrease in apoptosis in human gastric carcinomas.
胸苷磷酸化酶（dThdPase）/血小板衍生内皮细胞生长因子（PD - ECGF）在多种人类癌组织中的表达水平高于相邻正常组织。其高表达与肿瘤内微血管密度（IMVD）增加及患者预后不良相关。本研究探讨了dThdPase在人胃癌细胞凋亡、IMVD及p53表达中的作用。在34例早期癌中，12例（35.3%）检测到dThdPase表达；在36例进展期癌中，20例（55.6%）检测到dThdPase表达。从32例dThdPase阳性肿瘤（I类）中选取至少10个由弥漫性dThdPase表达的癌细胞组成的区域，从38例阴性肿瘤（II类）中选取10个无dThdPase表达的区域。早期胃癌中，I类癌的平均IMVD为88.8±19.4，II类癌为61.4±17.3；进展期胃癌中，I类癌的平均IMVD为98.8±21.0，II类癌为76.0±27.1。I类肿瘤的平均IMVD显著高于II类肿瘤（P<0.05）。早期胃癌中，I类癌的平均凋亡指数（AI：凋亡细胞百分比）为1.95±1.30，II类癌为3.76±1.49；进展期胃癌中，I类癌的平均AI为1.51±0.98，II类癌为2.14±0.66，无论肿瘤分期或组织学类型，dThdPase阴性肿瘤（II类）的平均AI值均显著高于阳性肿瘤（I类）（P<0.05）。AI与IMVD之间存在显著负相关（P<0.001）。这些结果表明，dThdPase表达与人胃癌肿瘤内微血管增加及细胞凋亡减少均相关。
