小鼠平滑肌肌动蛋白基因（Smtn）的结构与染色体定位

Structure and chromosome location of Smtn, the mouse smoothelin gene.

作者信息

Rensen S, Merkx G, Doevendans P, Geurts Van Kessel A, van Eys G

机构信息

Department of Molecular Cell Biology and Genetics, CARIM, University of Maastricht, Maastricht, The Netherlands.

出版信息

Cytogenet Cell Genet. 2000;89(3-4):225-9. doi: 10.1159/000015619.

DOI:10.1159/000015619

PMID:10965129

Abstract

Smoothelins are cytoskeleton-associated proteins that are found in contractile smooth muscle. Two isoforms have been identified: smoothelin-A, expressed in visceral tissues and smoothelin-B, found in vascular tissues. The mouse smoothelin gene (Smtn) was isolated and characterized. It was assigned to chromosome 11 band A2-A3 by fluorescence in situ hybridization. The gene consists of 20 exons and spans 23 kb. Its structure is conserved between mouse and human. The proximal promoter of both smoothelin-A and smoothelin-B contains several transcription factor-binding sites but lacks a consensus TATA box.

摘要

平滑肌肌动蛋白是一种与细胞骨架相关的蛋白质，存在于收缩性平滑肌中。已鉴定出两种亚型：在内脏组织中表达的平滑肌肌动蛋白-A和在血管组织中发现的平滑肌肌动蛋白-B。分离并鉴定了小鼠平滑肌肌动蛋白基因（Smtn）。通过荧光原位杂交将其定位到11号染色体A2-A3带。该基因由20个外显子组成，跨度为23 kb。其结构在小鼠和人类之间保守。平滑肌肌动蛋白-A和平滑肌肌动蛋白-B的近端启动子都包含几个转录因子结合位点，但缺乏共有TATA框。