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The problem of anabiosis or latent life: history and current concept.隐生现象或潜在生命问题：历史与当前概念
Proc R Soc Lond B Biol Sci. 1959 Mar 17;150(939):149-91. doi: 10.1098/rspb.1959.0013.
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Anhydrobiotic engineering.隐生工程
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Engineering desiccation tolerance in Escherichia coli.在大肠杆菌中构建干燥耐受性
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Osmotically induced intracellular trehalose, but not glycine betaine accumulation promotes desiccation tolerance in Escherichia coli.渗透诱导的细胞内海藻糖而非甘氨酸甜菜碱的积累可促进大肠杆菌的耐干燥性。
FEMS Microbiol Lett. 1999 May 1;174(1):57-63. doi: 10.1111/j.1574-6968.1999.tb13549.x.
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The role of vitrification in anhydrobiosis.玻璃化在隐生现象中的作用。
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Mannitol, a novel bacterial compatible solute in Pseudomonas putida S12.甘露醇，恶臭假单胞菌S12中一种新型的细菌相容性溶质。
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Role of the trehalose carrier in dehydration resistance of Saccharomyces cerevisiae.海藻糖载体在酿酒酵母抗脱水能力中的作用。
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The role of trehalose in the osmoadaptation of Escherichia coli NCIB 9484: interaction of trehalose, K+ and glutamate during osmoadaptation in continuous culture.海藻糖在大肠杆菌NCIB 9484渗透适应中的作用：连续培养渗透适应过程中海藻糖、钾离子和谷氨酸的相互作用
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革兰氏阴性菌的隐生工程

Anhydrobiotic engineering of gram-negative bacteria.

作者信息

García De Castro A, Bredholt H, Strøm A R, Tunnacliffe A

机构信息

Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT, United Kingdom.

出版信息

Appl Environ Microbiol. 2000 Sep;66(9):4142-4. doi: 10.1128/AEM.66.9.4142-4144.2000.

DOI:10.1128/AEM.66.9.4142-4144.2000
PMID:10966444
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC92274/
Abstract

Anhydrobiotic engineering aims to improve desiccation tolerance in living organisms by adopting the strategies of anhydrobiosis. This was achieved for Escherichia coli and Pseudomonas putida by osmotic induction of intracellular trehalose synthesis and by drying from trehalose solutions, resulting in long-term viability in the dried state.

摘要

隐生工程旨在通过采用隐生策略提高生物体的耐旱性。通过渗透诱导细胞内海藻糖合成以及从海藻糖溶液中干燥，大肠杆菌和恶臭假单胞菌实现了这一点，从而在干燥状态下具有长期生存能力。