多杂交展示系统的构建：携带两种外源黏附肽的鞭毛丝
Construction of a multihybrid display system: flagellar filaments carrying two foreign adhesive peptides.
作者信息
Tanskanen J, Korhonen T K, Westerlund-Wikström B
机构信息
Division of General Microbiology, Department of Biosciences, FIN-00014 University of Helsinki, Finland.
出版信息
Appl Environ Microbiol. 2000 Sep;66(9):4152-6. doi: 10.1128/AEM.66.9.4152-4156.2000.
Abstract
A multivalent, bifunctional flagellum carrying two different adhesive peptides in separate flagellin subunits within a filament was constructed in Escherichia coli. The inserted peptides were the fibronectin-binding 115-mer D repeat region of Staphylococcus aureus and the 302-mer collagen-binding region of YadA of Yersinia enterocolitica. Western blotting, immunoelectron microscopy, and adhesion tests with hybrid flagella from an in trans-complemented DeltafliC E. coli strain showed that individual filaments consisted of both recombinant flagellins.
摘要
在大肠杆菌中构建了一种多价、双功能鞭毛，该鞭毛在一根丝状体的不同鞭毛蛋白亚基中携带两种不同的粘附肽。插入的肽是金黄色葡萄球菌的纤连蛋白结合115聚体D重复区域和小肠结肠炎耶尔森菌YadA的302聚体胶原结合区域。对来自反式互补的ΔfliC大肠杆菌菌株的杂合鞭毛进行的蛋白质免疫印迹、免疫电子显微镜和粘附试验表明，单个丝状体由两种重组鞭毛蛋白组成。
相似文献
1
Construction of a multihybrid display system: flagellar filaments carrying two foreign adhesive peptides.
Appl Environ Microbiol. 2000 Sep;66(9):4152-6. doi: 10.1128/AEM.66.9.4152-4156.2000.
2
Simultaneous display of multiple foreign peptides in the FliD capping and FliC filament proteins of the Escherichia coli flagellum.
Appl Environ Microbiol. 2005 Aug;71(8):4263-8. doi: 10.1128/AEM.71.8.4263-4268.2005.
3
Functional expression of adhesive peptides as fusions to Escherichia coli flagellin.
Protein Eng. 1997 Nov;10(11):1319-26. doi: 10.1093/protein/10.11.1319.
4
Peptide display on bacterial flagella: principles and applications.
Int J Med Microbiol. 2000 Jul;290(3):223-30. doi: 10.1016/S1438-4221(00)80119-8.
5
Contribution of trimeric autotransporter C-terminal domains of oligomeric coiled-coil adhesin (Oca) family members YadA, UspA1, EibA, and Hia to translocation of the YadA passenger domain and virulence of Yersinia enterocolitica.
J Bacteriol. 2008 Jul;190(14):5031-43. doi: 10.1128/JB.00161-08. Epub 2008 May 16.
6
Characterization of adhesive epitopes with the OmpS display system.
Eur J Biochem. 2000 Jan;267(1):163-70. doi: 10.1046/j.1432-1327.2000.00981.x.
7
Matrix-binding proteins of Staphylococcus aureus: functional analysis of mutant and hybrid molecules.
Microbiology (Reading). 1999 Sep;145 ( Pt 9):2497-2505. doi: 10.1099/00221287-145-9-2497.
8
Discovery of a proteolytic flagellin family in diverse bacterial phyla that assembles enzymatically active flagella.
Nat Commun. 2017 Sep 12;8(1):521. doi: 10.1038/s41467-017-00599-0.
9
Functional mapping of the Yersinia enterocolitica adhesin YadA. Identification Of eight NSVAIG - S motifs in the amino-terminal half of the protein involved in collagen binding.
Mol Microbiol. 2000 Jul;37(1):192-206. doi: 10.1046/j.1365-2958.2000.01992.x.
10
Multiple Flagellin Proteins Have Distinct and Synergistic Roles in Agrobacterium tumefaciens Motility.
J Bacteriol. 2018 Nov 6;200(23). doi: 10.1128/JB.00327-18. Print 2018 Dec 1.
引用本文的文献
1
Discovery of a proteolytic flagellin family in diverse bacterial phyla that assembles enzymatically active flagella.
Nat Commun. 2017 Sep 12;8(1):521. doi: 10.1038/s41467-017-00599-0.
2
Simultaneous display of multiple foreign peptides in the FliD capping and FliC filament proteins of the Escherichia coli flagellum.
Appl Environ Microbiol. 2005 Aug;71(8):4263-8. doi: 10.1128/AEM.71.8.4263-4268.2005.
本文引用的文献
1
Peptide display on bacterial flagella: principles and applications.
Int J Med Microbiol. 2000 Jul;290(3):223-30. doi: 10.1016/S1438-4221(00)80119-8.
2
Secretion and assembly of regular surface structures in Gram-negative bacteria.
FEMS Microbiol Rev. 2000 Jan;24(1):21-44. doi: 10.1111/j.1574-6976.2000.tb00531.x.
3
Display of epitopes on the surface of tobacco mosaic virus: impact of charge and isoelectric point of the epitope on virus-host interactions.
J Mol Biol. 1999 Jul 2;290(1):9-20. doi: 10.1006/jmbi.1999.2860.
4
Intranuclear delivery of an antiviral peptide mediated by the B subunit of Escherichia coli heat-labile enterotoxin.
Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):5221-6. doi: 10.1073/pnas.96.9.5221.
5
Phage-display technology--finding a needle in a vast molecular haystack.
Curr Opin Biotechnol. 1999 Feb;10(1):87-93. doi: 10.1016/s0958-1669(99)80016-0.
6
Expression of thioredoxin random peptide libraries on the Escherichia coli cell surface as functional fusions to flagellin: a system designed for exploring protein-protein interactions.
Biotechnology (N Y). 1995 Apr;13(4):366-72. doi: 10.1038/nbt0495-366.
7
Functional expression of adhesive peptides as fusions to Escherichia coli flagellin.
Protein Eng. 1997 Nov;10(11):1319-26. doi: 10.1093/protein/10.11.1319.
8
Creating a bifunctional protein by insertion of beta-lactamase into the maltodextrin-binding protein.
Nat Biotechnol. 1997 Nov;15(12):1276-9. doi: 10.1038/nbt1197-1276.
9
Bacterial surface display: trends and progress.
Trends Biotechnol. 1997 May;15(5):185-92. doi: 10.1016/S0167-7799(97)01034-2.
10
Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines.
Nat Biotechnol. 1997 Jan;15(1):29-34. doi: 10.1038/nbt0197-29.
Zotero 插件