Georgiou G, Stathopoulos C, Daugherty P S, Nayak A R, Iverson B L, Curtiss R
Department of Chemical Engineering, University of Texas, Austin.
Nat Biotechnol. 1997 Jan;15(1):29-34. doi: 10.1038/nbt0197-29.
In recent years there has been considerable progress towards the development of expression systems for the display of heterologous polypeptides and, to a lesser extent, oligosaccharides on the surface of bacteria or yeast. The availability of protein display vectors has in turn provided the impetus for a range of exciting technologies. Polypeptide libraries can be displayed in bacteria and screened by cell sorting techniques, thus simplifying the isolation of proteins with high affinity for ligands. Expression of antigens on the surface of nonvirulent microorganisms is an attractive approach to the development of high-efficacy recombinant live vaccines. Finally, cells displaying protein receptors or antibodies are of use for analytical applications and bioseparations.
近年来，在开发用于在细菌或酵母表面展示异源多肽以及在较小程度上展示寡糖的表达系统方面取得了相当大的进展。蛋白质展示载体的可用性反过来又为一系列令人兴奋的技术提供了动力。多肽文库可以在细菌中展示并通过细胞分选技术进行筛选，从而简化了对配体具有高亲和力的蛋白质的分离。在无毒微生物表面表达抗原是开发高效重组活疫苗的一种有吸引力的方法。最后，展示蛋白质受体或抗体的细胞可用于分析应用和生物分离。
