Certik M, Shimizu S
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
Appl Microbiol Biotechnol. 2000 Aug;54(2):224-30. doi: 10.1007/s002530000361.
Kinetic analysis of arachidonic acid (AA)-oil biosynthesis by Mortierella alpina 1S-4 growing under lipid-accumulating (LN medium) and non-lipid-accumulating (HN medium) conditions was investigated and compared with industrial AA fermentation. Various kinetic parameters of these cultivation processes demonstrate a characteristic pattern of the lipogenesis in this fungus, where growth phase, phase of oil accumulation and phase of AA synthesis are distinct from each other. The fungus utilizing LN medium synthesized 32.3 g fatty acid 100 g(-1) glucose on the 4th day of cultivation and reached the maximum daily fatty acid accumulation (expressed as differential specific rate qD(FA/B)) of 9.5%. Our results also indicate that a qD(FA/B) value of about 2.5% might be critical for lipid overproduction in M. alpina. AA was rapidly incorporated into triacylglycerols (90% of total AA) at the later cultivation phase and overall AA yield was directly related to the total yield of fatty acid.
研究了高山被孢霉1S-4在脂质积累条件（LN培养基）和非脂质积累条件（HN培养基）下生长时花生四烯酸（AA）-油脂生物合成的动力学分析，并与工业AA发酵进行了比较。这些培养过程的各种动力学参数表明了这种真菌中脂肪生成的特征模式，其中生长阶段、油脂积累阶段和AA合成阶段彼此不同。利用LN培养基的真菌在培养第4天合成了32.3 g脂肪酸/100 g葡萄糖，并达到了9.5%的最大日脂肪酸积累量（以差异比速率qD(FA/B)表示）。我们的结果还表明，约2.5%的qD(FA/B)值可能是高山被孢霉脂质过量生产的关键。在培养后期，AA迅速掺入三酰甘油（占总AA的90%），AA的总产量与脂肪酸的总产量直接相关。
