核苷酸诱发的正常及多汗症患者人汗腺细胞中的离子转运和胞内钙离子浓度变化

Nucleotide-evoked ion transport and [Ca(2+)](i) changes in normal and hyperhidrotic human sweat gland cells.

作者信息

Bovell D L, Clunes M T, Elder H Y, Wong C H, Ko W H

机构信息

School of Biological and Biomedical Sciences, Glasgow Caledonian University, G4 0BA, Glasgow, UK.

出版信息

Eur J Pharmacol. 2000 Sep 1;403(1-2):45-8. doi: 10.1016/s0014-2999(00)00570-7.

DOI:10.1016/s0014-2999(00)00570-7

PMID:10969142

Abstract

Apical and basolateral application of ATP and UTP evoked Ca(2+) and short circuit current (Isc) increases in normal and hyperhidrotic human eccrine sweat gland cells grown into functionally polarised epithelia on permeable supports. Basolateral application to hyperhidrotic cells exhibited a markedly greater increase in Isc than in normal cells. Hyperhidrotic cells also demonstrated differences from the normal in Ca(2+) and Isc responses to ATP when pre-treated with thapsigargin. The data demonstrate the presence of apical and basolateral receptors that allow nucleotides to increase Ca(2+) and Isc. The results suggest that changes from the normal in transepithelial ion transport contribute to the characteristic excessive fluid production of hyperhidrotic sweat glands.

摘要

在可渗透支持物上生长为功能极化上皮的正常和多汗症人外泌汗腺细胞中，顶端和基底外侧应用ATP和UTP可引起细胞内钙离子浓度（Ca(2+)）和短路电流（Isc）增加。向多汗症细胞基底外侧应用时，Isc的增加明显大于正常细胞。当用毒胡萝卜素预处理时，多汗症细胞在Ca(2+)和对ATP的Isc反应方面也表现出与正常细胞的差异。数据表明存在顶端和基底外侧受体，使核苷酸能够增加Ca(2+)和Isc。结果表明，跨上皮离子转运与正常情况的改变导致了多汗症汗腺特征性的过多液体分泌。