Department of Biology, University of Copenhagen, Denmark.
Pancreas. 2013 Apr;42(3):452-60. doi: 10.1097/MPA.0b013e318264c302.
The objective of the study was to establish a solid model of polarized epithelium for human pancreatic ducts, where electrical parameters could be measured as indicators of ion transport. Further, we aimed to determine functional expression of several receptors, in particular, purinergic receptors, and determine their effects on ion transport.
Human adenocarcinoma cell line Capan-1 cells were grown on permeable supports and set in Ussing chambers for electrophysiological recordings. Transepithelial voltage (Vte), resistance, and short-circuit currents (Isc) were measured in response to agonists.
Secretin, vasoactive intestinal peptide (VIP), acetylcholine, forskolin, ionomycin, adenosine 5'-triphosphate (ATP), uridine 5'-triphosphate (UTP), 3'-O-(4-benzoyl)benzoyl ATP, and adenosine induced lumen negative Vte and Isc. These changes were consistent with anion secretion, as verified in forskolin-stimulated preparations. Extracellular nucleotides, ATP, and UTP, applied from luminal and basolateral sides, caused largest responses: Vte increased up to -5 mV, Isc increased to 20 to 30 μA/cm, and resistance decreased by up to 200 Ω·cm.
Transepithelial transport in human pancreatic duct epithelium, Capan-1 cells, is regulated by secretin, VIP, acetylcholine, adenosine, and purinergic P2 receptors; and this human model has a good potential for studies of physiology and pathophysiology of pancreatic duct ion transport.
本研究旨在建立人胰腺导管的极化上皮的固体模型，其中可以测量电参数作为离子转运的指标。此外，我们旨在确定几种受体的功能表达，特别是嘌呤能受体，并确定它们对离子转运的影响。
将人腺癌细胞系 Capan-1 细胞在可渗透的载体上生长，并置于 Ussing 室中进行电生理记录。测量对激动剂的跨上皮电压（Vte）、电阻和短路电流（Isc）。
促胰液素、血管活性肠肽（VIP）、乙酰胆碱、forskolin、离子霉素、三磷酸腺苷（ATP）、三磷酸尿苷（UTP）、3'-O-（4-苯甲酰）苯甲酰基 ATP 和腺苷诱导管腔负 Vte 和 Isc。这些变化与阴离子分泌一致，在 forskolin 刺激的制剂中得到验证。从腔侧和基底外侧施加细胞外核苷酸、ATP 和 UTP 引起最大的反应：Vte 增加高达-5 mV，Isc 增加到 20 至 30 μA/cm，电阻降低高达 200 Ω·cm。
Capan-1 细胞人胰腺导管上皮的跨上皮转运受促胰液素、VIP、乙酰胆碱、腺苷和嘌呤能 P2 受体调节；并且该人模型具有研究胰腺导管离子转运的生理学和病理生理学的良好潜力。
