Korolev Iu N
Biofizika. 1975 Mar-Apr;20(2):271-5.
The problems are considered of the determination of the optical constants (dispersion of refraction, n2, and absorption, K, indexes) of strongly dispersing biological objects on undestroyed cells of microorganisms (E. coli, strain C-85) by the method of repeated attenuated total refraction. The volume of unpacked intercellular space and the effect of repeated propagation of the light current through measuring elements with high refraction index were taken into account. Relationships R, s (n1, n2, O) K were obtained for determining the optical constants on the elements with the angle of light current incidence 0=45 degrees, made of infrared glass, IRG-24, IRF-24 and of Si and Ge as well. The optical constants of bacteria in the region of absorption bands 1660, 1550 cm-1 were also determined.
研究了通过重复衰减全折射法测定强分散性生物物体（大肠杆菌C - 85菌株）未破坏细胞的光学常数（折射色散n2和吸收系数K）的问题。考虑了未填充细胞间空间的体积以及光电流在具有高折射率的测量元件中重复传播的影响。获得了在光电流入射角θ = 45度时，由红外玻璃IRG - 24、IRF - 24以及硅和锗制成的元件上测定光学常数的关系R、s（n1、n2、θ）、K。还测定了细菌在1660、1550 cm-1吸收带区域的光学常数。
