[从微生物中分离三磷酸腺苷的技术]
[Technics for isolation of ATP from microorganisms].
作者信息
Aksenov S I, Sosunova L N
出版信息
Mikrobiologiia. 1975 Nov-Dec;44(6):1107-11.
PMID:765686
Abstract
A technique for isolation of ATP from microorganisms is suggested. The technique is based on increasing permeability of the cell membrane for substances of low molecular weight during dehydration. Treatment of the dry biomass with boiling water gives a higher yield of ATP from the cells of eukaryote and prokaryote microorganisms as compared with other techniques, and permits to register the amount of APT at the moment of experiment.
摘要
提出了一种从微生物中分离三磷酸腺苷（ATP）的技术。该技术基于在脱水过程中增加细胞膜对低分子量物质的通透性。与其他技术相比，用沸水处理干燥的生物质能从真核生物和原核生物微生物细胞中获得更高产量的ATP，并能在实验时记录三磷酸腺苷（ATP）的含量。
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引用本文的文献
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Fluorometric determination of adenosine nucleotide derivatives as measures of the microfouling, detrital, and sedimentary microbial biomass and physiological status.荧光法测定腺苷核苷酸衍生物作为微污染、碎屑和沉积物微生物生物量和生理状态的测量指标。
Appl Environ Microbiol. 1980 Sep;40(3):539-48. doi: 10.1128/aem.40.3.539-548.1980.
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