Bruch J, Schulz W A, Häussler J, Melzner I, Brüderlein S, Möller P, Kemmerling R, Vogel W, Hameister H
Department of Medical Genetics, University Ulm, Germany.
Cancer Res. 2000 Aug 15;60(16):4526-30.
Eight cell lines from transitional cell carcinoma of the urinary bladder were analyzed by comparative genomic hybridization. All tumor lines exhibited frequent chromosome gains (11.5/cell line) and losses (8.4/cell line). In six cell lines, gain of chromosome 5p was associated with gains of 6p and 20q. In five of these cell lines, amplification of parts of 6p was observed. Cytogenetic investigation combined with fluorescence in situ hybridization analysis revealed typical marker chromosomes with homogeneously staining regions (HSRs) containing material from 6p. By hybridizing individual yeast artificial chromosome probes from a chromosome 6p contig to these HSRs, a contig of three yeast artificial chromosomes common to all 6p HSRs was identified that spans less than 2 Mb. The genes SOX4 and PRL were shown to map to this region and to be coamplified in the cell lines. However, SOX4 was not overexpressed in any cell line and PRL was not expressed at all. Thus, the presumptive 6p oncogene remains to be conclusively identified.
通过比较基因组杂交分析了来自膀胱移行细胞癌的8个细胞系。所有肿瘤细胞系均频繁出现染色体增加（每个细胞系11.5条）和丢失（每个细胞系8.4条）。在6个细胞系中，5号染色体短臂的增加与6号染色体短臂和20号染色体长臂的增加相关。在其中5个细胞系中，观察到6号染色体短臂部分区域的扩增。细胞遗传学研究结合荧光原位杂交分析发现了典型的标记染色体，其具有包含6号染色体短臂物质的均匀染色区（HSR）。通过将来自6号染色体短臂重叠群的单个酵母人工染色体探针与这些HSR杂交，鉴定出了所有6号染色体HSR共有的由3个酵母人工染色体组成的重叠群，其跨度小于2 Mb。基因SOX4和PRL定位于该区域并在细胞系中共同扩增。然而，SOX4在任何细胞系中均未过度表达，PRL根本不表达。因此，推定的6号染色体短臂上的致癌基因仍有待最终确定。
