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A new phosphospecific cell-based ELISA for p42/p44 mitogen-activated protein kinase (MAPK), p38 MAPK, protein kinase B and cAMP-response-element-binding protein.一种用于p42/p44丝裂原活化蛋白激酶（MAPK）、p38 MAPK、蛋白激酶B和环磷酸腺苷反应元件结合蛋白的新型基于细胞的磷酸特异性酶联免疫吸附测定法。
Biochem J. 2000 Sep 15;350 Pt 3(Pt 3):717-22.
2
Genetic deletion of PKR abrogates TNF-induced activation of IkappaBalpha kinase, JNK, Akt and cell proliferation but potentiates p44/p42 MAPK and p38 MAPK activation.PKR的基因缺失消除了肿瘤坏死因子诱导的IκBα激酶、JNK、Akt激活以及细胞增殖，但增强了p44/p42丝裂原活化蛋白激酶（MAPK）和p38 MAPK的激活。
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3
Proinsulin C-peptide activates cAMP response element-binding proteins through the p38 mitogen-activated protein kinase pathway in mouse lung capillary endothelial cells.胰岛素原C肽通过p38丝裂原活化蛋白激酶途径激活小鼠肺毛细血管内皮细胞中的环磷酸腺苷反应元件结合蛋白。
Biochem J. 2002 Sep 15;366(Pt 3):737-44. doi: 10.1042/BJ20020344.
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IGF-I and vasoactive intestinal peptide (VIP) regulate cAMP-response element-binding protein (CREB)-dependent transcription via the mitogen-activated protein kinase (MAPK) pathway in pituitary cells: requirement of Rap1.胰岛素样生长因子-I（IGF-I）和血管活性肠肽（VIP）通过垂体细胞中的丝裂原活化蛋白激酶（MAPK）途径调节环磷酸腺苷反应元件结合蛋白（CREB）依赖性转录：Rap1的需求。
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Activation of extracellular signal-regulated kinase and c-Jun-NH(2)-terminal kinase but not p38 mitogen-activated protein kinases is required for RRR-alpha-tocopheryl succinate-induced apoptosis of human breast cancer cells.RRR-α-生育酚琥珀酸酯诱导人乳腺癌细胞凋亡需要细胞外信号调节激酶和c-Jun氨基末端激酶的激活，而p38丝裂原活化蛋白激酶则不需要。
Cancer Res. 2001 Sep 1;61(17):6569-76.
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Active, phosphorylation-dependent MAP kinases, MAPK/ERK, SAPK/JNK and p38, and specific transcription factor substrates are differentially expressed following systemic administration of kainic acid to the adult rat.在成年大鼠全身给予海藻酸后，活性的、磷酸化依赖性丝裂原活化蛋白激酶（MAPK）、MAPK/细胞外信号调节激酶（ERK）、应激激活蛋白激酶/应激活化蛋白激酶（SAPK/JNK）和p38以及特定转录因子底物呈现出差异表达。
Acta Neuropathol. 2002 Apr;103(4):391-407. doi: 10.1007/s00401-001-0481-9. Epub 2002 Jan 31.
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High K+ and IGF-1 protect cerebellar granule neurons via distinct signaling pathways.高钾离子和胰岛素样生长因子-1通过不同的信号通路保护小脑颗粒神经元。
J Neurosci Res. 2004 Mar 15;75(6):794-806. doi: 10.1002/jnr.20024.
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Adenosine stimulates CREB activation in macrophages via a p38 MAPK-mediated mechanism.腺苷通过p38丝裂原活化蛋白激酶介导的机制刺激巨噬细胞中的CREB激活。
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Guanine nucleotide exchange factor Epac2-dependent activation of the GTP-binding protein Rap2A mediates cAMP-dependent growth arrest in neuroendocrine cells.鸟嘌呤核苷酸交换因子Epac2依赖的GTP结合蛋白Rap2A激活介导神经内分泌细胞中cAMP依赖的生长停滞。
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Improved intra-array and interarray normalization of peptide microarray phosphorylation for phosphorylome and kinome profiling by rational selection of relevant spots.通过合理选择相关斑点改进肽微阵列磷酸化的阵列内和阵列间归一化，用于磷酸化蛋白质组和激酶组分析。
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Modeling long-term host cell-Giardia lamblia interactions in an in vitro co-culture system.在体外共培养系统中模拟宿主细胞与蓝氏贾第鞭毛虫的长期相互作用。
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本文引用的文献

1
Prostaglandin E2 increases proenkephalin mRNA level in rat astrocyte-enriched culture.前列腺素E2可提高大鼠富含星形胶质细胞培养物中前脑啡肽原mRNA的水平。
Brain Res Mol Brain Res. 1998 Oct 1;60(2):203-14. doi: 10.1016/s0169-328x(98)00182-x.
2
Macrophages induce cellular immunity by activating Th1 cell responses and suppressing Th2 cell responses.巨噬细胞通过激活Th1细胞反应和抑制Th2细胞反应来诱导细胞免疫。
J Immunol. 1998 Jun 1;160(11):5300-8.
3
Comparison of the roles of mitogen-activated protein kinase kinase and phosphatidylinositol 3-kinase signal transduction in neutrophil effector function.丝裂原活化蛋白激酶激酶和磷脂酰肌醇3激酶信号转导在中性粒细胞效应功能中的作用比较
Biochem J. 1998 Jan 1;329 ( Pt 1)(Pt 1):121-30. doi: 10.1042/bj3290121.
4
The PI 3-kinase/Akt signaling pathway delivers an anti-apoptotic signal.磷脂酰肌醇-3激酶/蛋白激酶B信号通路传递抗凋亡信号。
Genes Dev. 1997 Mar 15;11(6):701-13. doi: 10.1101/gad.11.6.701.
5
Mechanism of activation of protein kinase B by insulin and IGF-1.胰岛素和胰岛素样生长因子-1激活蛋白激酶B的机制。
EMBO J. 1996 Dec 2;15(23):6541-51.
6
Phosphorylation of CREB in ovine pars tuberalis is regulated both by cyclic AMP-dependent and cyclic AMP-independent mechanisms.绵羊结节部中CREB的磷酸化受环磷酸腺苷依赖性和环磷酸腺苷非依赖性机制的调控。
J Neuroendocrinol. 1996 Aug;8(8):635-45.
7
Impaired cAMP-mediated gene expression and decreased cAMP response element binding protein in senescent cells.衰老细胞中cAMP介导的基因表达受损及cAMP反应元件结合蛋白减少。
Am J Physiol. 1996 Jul;271(1 Pt 1):C362-71. doi: 10.1152/ajpcell.1996.271.1.C362.
8
Nuclear protein CBP is a coactivator for the transcription factor CREB.核蛋白CBP是转录因子CREB的一种共激活因子。
Nature. 1994 Jul 21;370(6486):223-6. doi: 10.1038/370223a0.
9
Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction.磷脂酰肌醇-3-羟基激酶信号转导中的蛋白激酶B（c-Akt）
Nature. 1995 Aug 17;376(6541):599-602. doi: 10.1038/376599a0.
10
PD 098059 is a specific inhibitor of the activation of mitogen-activated protein kinase kinase in vitro and in vivo.PD 098059在体外和体内均是丝裂原活化蛋白激酶激酶激活的特异性抑制剂。
J Biol Chem. 1995 Nov 17;270(46):27489-94. doi: 10.1074/jbc.270.46.27489.

一种用于p42/p44丝裂原活化蛋白激酶（MAPK）、p38 MAPK、蛋白激酶B和环磷酸腺苷反应元件结合蛋白的新型基于细胞的磷酸特异性酶联免疫吸附测定法。

A new phosphospecific cell-based ELISA for p42/p44 mitogen-activated protein kinase (MAPK), p38 MAPK, protein kinase B and cAMP-response-element-binding protein.

作者信息

Versteeg H H, Nijhuis E, van den Brink G R, Evertzen M, Pynaert G N, van Deventer S J, Coffer P J, Peppelenbosch M P

机构信息

Laboratory for Experimental Internal Medicine, G2-130, Academic Medical Centre, Meibergdreef 9, NL-1105 AZ Amsterdam, The Netherlands.

出版信息

Biochem J. 2000 Sep 15;350 Pt 3(Pt 3):717-22.

PMID:10970784
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1221302/
Abstract

Assaying activation of signal transduction is laborious and does not allow the study of large numbers of samples, essential for high-throughput drug screens or for large groups of patients. Using phosphospecific antibodies, we have developed ELISA techniques enabling non-radioactive semi-quantitative assessment of the activation state of p42/p44 mitogen-activated protein kinase (MAPK), p38 MAPK, protein kinase B and the transcription factor cAMP-response-element-binding protein (CREB) in 96-well plates. This assay has been termed PACE (phosphospecific antibody cell-based ELISA) and was used successfully for both adherent and suspension cells. Various stimuli induced dose-dependent enzymic activity of which the kinetics closely correlated with those measured via classical methodology. Using PACE we have now characterized for the first time the concentration-dependent effects of various inflammatory prostaglandins on CREB phosphorylation in macrophages. PACE is a straightforward and novel technique enabling the large-scale analysis of signal transduction.

摘要

检测信号转导的激活过程既费力又无法对大量样本进行研究，而这对于高通量药物筛选或大量患者群体来说至关重要。我们利用磷酸特异性抗体开发了酶联免疫吸附测定（ELISA）技术，能够在96孔板中对p42/p44丝裂原活化蛋白激酶（MAPK）、p38 MAPK、蛋白激酶B和转录因子环磷酸腺苷反应元件结合蛋白（CREB）的激活状态进行非放射性半定量评估。该检测方法被称为PACE（基于磷酸特异性抗体的细胞酶联免疫吸附测定），已成功应用于贴壁细胞和悬浮细胞。各种刺激诱导了剂量依赖性酶活性，其动力学与通过经典方法测得的结果密切相关。利用PACE，我们首次表征了各种炎性前列腺素对巨噬细胞中CREB磷酸化的浓度依赖性影响。PACE是一种简单新颖的技术，能够对信号转导进行大规模分析。