Vesselinovitch S D, Kyriazis A P, Mihailovich N, Rao K V
Cancer Res. 1975 Aug;35(8):1963-9.
The response of lymphoreticular tissues to a single i.p. injection of benzo(a)pyrene was studied in the first generation of C57BL/6J X C3HeB/FeJ F1 and C3HeB/FeJ X A/J F1 mice. Groups of 1-, 15-, and 42-day-old animals of both sexes received 75 or 150 mug of the carcinogen per g body weight. After a period of approximately 90 weeks, a high incidence (up to 43%) of reticulum cell sarcomas was observed in C57BL/6J X C3HeB/FeJ F1 mice treated with benzo(a)pyrene at 40 days of age. Animals treated with carcinogen at younger ages had a lower incidence of reticulum cell sarcomas. These sarcomas showed marked cellular pleomorphism and were classified into histiocytic, epitheloid-nodular, reticulocytic, and fibrocytic forms according to the predominant cell type. Lymphomas of thymic and extrathymic lymphoid origin and leukemia of granulocytic type were seen in a descending order of frequency. Control animals of either strain that were killed at 90 weeks of age were basically free of lymphoreticular tumors, while those kept under observation up to 170 weeks developed these tumors in 24% (C57BL X C3H F1) and 10% (C3H X A/J F1), respectively. Studies revealed that the augmentation and/or acceleration of development of the lymphoreticular neoplasms and specifically reticulum cell sarcomas by benzo(a)pyrene was dependent upon the strain and sex of mice used and the age at which the animals were exposed to carcinogen.
在第一代C57BL/6J×C3HeB/FeJ F1和C3HeB/FeJ×A/J F1小鼠中，研究了淋巴网状组织对单次腹腔注射苯并(a)芘的反应。给1日龄、15日龄和42日龄的雌雄动物每组注射每克体重75或150微克的致癌物。经过约90周的时间，在40日龄时用苯并(a)芘处理的C57BL/6J×C3HeB/FeJ F1小鼠中观察到网状细胞肉瘤的高发病率（高达43%）。在较年轻时用致癌物处理的动物中，网状细胞肉瘤的发病率较低。这些肉瘤表现出明显的细胞多形性，并根据主要细胞类型分为组织细胞型、上皮样结节型、网状细胞型和成纤维细胞型。胸腺和胸腺外淋巴起源的淋巴瘤以及粒细胞型白血病的发生频率依次降低。在90周龄时处死的任一品系对照动物基本没有淋巴网状肿瘤，而那些观察至170周的动物中，C57BL×C3H F1和C3H×A/J F1分别有24%和10%发生了这些肿瘤。研究表明，苯并(a)芘对淋巴网状肿瘤尤其是网状细胞肉瘤的发生发展的增强和/或加速作用取决于所用小鼠的品系和性别以及动物接触致癌物时的年龄。
