Vesselinovitch S D, Mihailovich N, Rao K V
Cancer Res. 1978 Jul;38(7):2003-10.
The metastatic capabilities of well-defined nodular hepatic lesions induced by benzo(a)pyrene, ethylnitrosourea, benzidine.2HCl, and diethylnitrosamine were evaluated. Coded liver and lung tissues from 1264 treated C57BL/6J x C3HeB/FeJ F1 mice were assessed independently for the presence of primary nodular lesions and metastases, respectively. Primary lesions were classified according to their size, cell morphology, and growth patterns into hyperplastic, adenomatous, and trabecular nodules. None of the 126 mice bearing hyperplastic nodules had pulmonary metastases. Four of 291 (1.4%) mice with adenomatous nodular lesions showed metastases. In contrast, of the 733 mice bearing the trabecular type of nodular lesions alone or in combination with other lesions 266 (36%) showed pulmonary metastases. The pulmonary metastases were first detected in mice dying between 51 and 60 weeks of age (5%). This rate increased as a function of age at death, reaching an incidence of 51% in mice surviving more than 81 weeks. It was concluded that nodules showing trabecular and the more anaplastic solid sheet type of growths represented bona fide hepatocellular carcinomas in the mouse.
评估了由苯并(a)芘、乙基亚硝基脲、盐酸联苯胺和二乙基亚硝基胺诱导产生的明确的结节性肝损伤的转移能力。分别对1264只经处理的C57BL/6J×C3HeB/FeJ F1小鼠的编码肝脏和肺组织进行独立评估，以确定原发性结节性损伤和转移灶的存在情况。原发性损伤根据其大小、细胞形态和生长模式分为增生性、腺瘤性和小梁状结节。126只患有增生性结节的小鼠均无肺转移。291只患有腺瘤性结节性损伤的小鼠中有4只(1.4%)出现转移。相比之下，在733只仅患有小梁状结节性损伤或与其他损伤合并存在的小鼠中，有266只(36%)出现肺转移。肺转移首先在51至60周龄死亡的小鼠中被检测到(5%)。该发生率随死亡年龄增加而升高，在存活超过81周的小鼠中发生率达到51%。得出的结论是，表现为小梁状以及更间变的实性片状生长的结节代表小鼠真正的肝细胞癌。
