Kääb M J, Ito K, Rahn B, Clark J M, Nötzli H P
Charité, Department for Trauma and Reconstructive Surgery, Humboldt University, Berlin, Germany.
Cells Tissues Organs. 2000;167(2-3):106-20. doi: 10.1159/000016774.
Little is known about the morphological effect of a mechanical load upon articular cartilage. The objective of this study was to describe and quantify the deformation of the articular cartilage collagen structure of the tibial plateau under static loading. Whole intact rabbit knee joints were loaded in vitro by simulating a quadriceps force of 3x, 1x or 0.5x body weight (high, medium, low) over durations of 30 or 5 min (long, short). Specimens were cryopreserved while under load and prepared for morphological evaluation by field emission scanning electron microscopy. Under high force and long duration loading the collagen fibers exhibited high deformation with an increased thickness of the layer of collagen fibers oriented almost parallel to the surface and a cartilage thickness reduced to 54%. Collagen fiber deformation occurred mostly in the transitional and upper radial zone. The area of tibial indentation and the cartilage thickness reduction increased with magnitude and duration of load. The collagen matrix did show a bulging edge at the border of the meniscus and exhibited remarkable deformation under the meniscus.
关于机械负荷对关节软骨的形态学影响，人们了解甚少。本研究的目的是描述和量化静态负荷下胫骨平台关节软骨胶原结构的变形情况。通过模拟相当于体重3倍、1倍或0.5倍（高、中、低）的股四头肌力量，对完整的兔膝关节进行体外加载，持续时间为30分钟或5分钟（长、短）。标本在加载状态下进行冷冻保存，并通过场发射扫描电子显微镜进行形态学评估准备。在高负荷和长时间加载下，胶原纤维表现出高度变形，几乎与表面平行排列的胶原纤维层厚度增加，软骨厚度减少至54%。胶原纤维变形主要发生在过渡区和上放射状区。胫骨压痕面积和软骨厚度减少随着负荷大小和持续时间的增加而增加。胶原基质在半月板边缘确实显示出凸起边缘，并且在半月板下方表现出明显变形。
