Metallic copper-induced granulocyte exudation in the study of granulocytopoiesis.

C3H/HeHa mice implanted with rods of metallic copper (CR) or glass (GR) exudate neutrophil granulocytes and mononuclear cells into the site of rod implant (peritoneal cavity). Exudation in CR mice is substantially greater than in GR animals. In CR mice there is an impressive stimulation of myelopoiesis measured in the femoral marrow subsequent to the initial accumulation in the peritoneal cavity. Serum levels of colony stimulating activity (CSA), an in vitro myeloproliferative stimulating activity, are elevated in such animals, as are femoral marrow agarcolony forming cells (CFC). The procedures is useful to the study of myelopoiesis and myelopoietic regulatory mechanisms.