Burgaleta C, Golde D W
Cancer Res. 1977 Jun;37(6):1739-42.
Glucan, a potent reticuloendothelial stimulant, is a glucopyranose polysaccharide derived from zymosan. Because of glucan's potential as an immunotherapeutic agent, we performed studies in order to determine its effect on granulopoiesis and macrophage production in mice. One week after the i.p. injection of 4 mg of glucan, there was a tenfold increase in colony-forming cells in the spleen and approximately a twofold increment of cells in the bone marrow and the peritoneal cavity capable of colony formation in vitro. There was a relative and absolute increase in the number of pure macrophage colonies from bone marrow and spleen. The total macrophage content in spleen, peritoneal cavity, and bone marrow as also increased in the treated mice. Serum from glucan-injected mice had high colony-stimulating activity levels, and the peritoneal macrophages elaborated increased colony-stimulating activity in vitro as compared to controls. Peripheral white blood cell counts were two times greater than those of control in the glucan-treated mice. These studies indicate that glucan administration results in increased granulocyte and macrophage production. The enhanced leukopoiesis is probably mediated in part by augmented release of colony-stimulating activity from macrophages. These observations suggest that the use of glucan as an immunotherapeutic agent can result in an increased number of available effector cells.
葡聚糖是一种有效的网状内皮系统刺激剂，是一种源自酵母聚糖的吡喃葡萄糖多糖。鉴于葡聚糖作为一种免疫治疗剂的潜力，我们开展了研究以确定其对小鼠粒细胞生成和巨噬细胞产生的影响。腹腔注射4毫克葡聚糖一周后，脾脏中集落形成细胞增加了10倍，骨髓和腹腔中能够在体外形成集落的细胞数量增加了约2倍。骨髓和脾脏中纯巨噬细胞集落的数量有相对和绝对的增加。经处理的小鼠脾脏、腹腔和骨髓中的巨噬细胞总量也有所增加。注射葡聚糖的小鼠血清具有较高的集落刺激活性水平，与对照组相比，腹腔巨噬细胞在体外产生的集落刺激活性有所增加。葡聚糖处理的小鼠外周白细胞计数是对照组的两倍。这些研究表明，给予葡聚糖会导致粒细胞和巨噬细胞生成增加。白细胞生成的增强可能部分是由巨噬细胞集落刺激活性释放增加介导的。这些观察结果表明，使用葡聚糖作为免疫治疗剂可导致可用效应细胞数量增加。
