Matsui E, Kaneko H, Teramoto T, Fukao T, Inoue R, Kasahara K, Takemura M, Seishima M, Kondo N
Department of Paediatrics; Department of Clinical Laboratory, Gifu University School of Medicine, Japan.
Clin Exp Allergy. 2000 Sep;30(9):1250-6. doi: 10.1046/j.1365-2222.2000.00931.x.
Several studies have shown that interleukin (IL)-4 and interferon-gamma (IFNgamma) are important for the regulation of IgE production and that IL-12 induces IFNgamma.
IFNgamma production in response to IL-12 stimulation and IL-12 production were investigated in peripheral blood mononuclear cells (PBMCs) of atopic patients with various levels of serum IgE.
Cytokine production from PBMCs was measured following stimulation with a nonspecific stimulator (phytohemagglutinin: PHA), a specific stimulator (Der f 1) and IL-12 in 17 healthy controls and 23 atopic patients with various serum IgE levels.
The IFNgamma production by IL-12-stimulated PBMCs in the atopic group was lower than that in the control group. Furthermore, the serum IgE level was negatively correlated with IFNgamma production by PBMCs stimulated with IL-12 (P < 0.001), and with IL-12 production by PBMCs stimulated with Der f 1 (P < 0.001). Although the IFNgamma concentrations by PHA-stimulated PBMCs were correlated with those by IL-12-stimulated PBMCs, there were differences in several patients.
Our results indicated that atopic patients may have some abnormality in the IL-12-IFNgamma loop. It was shown that the elevation in IgE levels in atopic patients may be due to reduced IFNgamma production in response to IL-12 stimulation and/or due to reduced IL-12 production.
多项研究表明，白细胞介素（IL）-4和干扰素-γ（IFNγ）对IgE产生的调节很重要，且IL-12可诱导IFNγ。
研究不同血清IgE水平的特应性患者外周血单个核细胞（PBMC）对IL-12刺激的IFNγ产生情况及IL-12的产生情况。
在17名健康对照者和23名不同血清IgE水平的特应性患者中，用非特异性刺激物（植物血凝素：PHA）、特异性刺激物（Der f 1）和IL-12刺激PBMC后，检测细胞因子的产生。
特应性组中IL-12刺激的PBMC产生的IFNγ低于对照组。此外，血清IgE水平与IL-12刺激的PBMC产生的IFNγ呈负相关（P<0.001），与Der f 1刺激的PBMC产生的IL-12呈负相关（P<0.001）。虽然PHA刺激的PBMC产生的IFNγ浓度与IL-12刺激的PBMC产生的IFNγ浓度相关，但在一些患者中存在差异。
我们的结果表明，特应性患者的IL-12-IFNγ环路可能存在某些异常。结果显示，特应性患者IgE水平升高可能是由于对IL-12刺激的IFNγ产生减少和/或由于IL-12产生减少所致。
