Shikano H, Kato Z, Kaneko H, Watanabe M, Inoue R, Kasahara K, Takemura M, Kondo N
Department of Paediatrics, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu 500-8705, Japan.
Clin Exp Allergy. 2001 Aug;31(8):1263-70. doi: 10.1046/j.1365-2222.2001.01141.x.
Several studies have shown that interleukin (IL)-4 and interferon-gamma (IFN-gamma) are important for the regulation of immunoglobulin E (IgE) production and that IL-18 and IL-12 induce IFN-gamma.
IFN-gamma production in response to IL-18 or IL-12 stimulation was investigated in peripheral blood mononuclear cells (PBMCs) of atopic patients with various levels of serum IgE.
Cytokine production from PBMCs was measured following stimulation with a non-specific stimulator (phytohemagglutinin: PHA), IL-18 or IL-12 in 12 healthy controls and 26 atopic patients with various serum IgE levels.
IFN-gamma production by IL-18-stimulated PBMCs was positively correlated with IFN-gamma production by IL-12-stimulated PBMCs (P < 0.05). However some atopic patients showed discrepancy between the levels of IFN-gamma production stimulated by IL-12 and by IL-18.
The results shown here suggest the presence of abnormalities in the IL-12 and/or IL-18 signalling pathways, such as genetic defects in the atopic patients.
多项研究表明，白细胞介素（IL）-4和干扰素-γ（IFN-γ）对免疫球蛋白E（IgE）的产生调节很重要，且IL-18和IL-12可诱导IFN-γ。
在不同血清IgE水平的特应性患者外周血单个核细胞（PBMC）中，研究对IL-18或IL-12刺激的IFN-γ产生情况。
在12名健康对照者和26名不同血清IgE水平的特应性患者中，用非特异性刺激物（植物血凝素：PHA）、IL-18或IL-12刺激PBMC后，检测细胞因子的产生。
IL-18刺激的PBMC产生的IFN-γ与IL-12刺激的PBMC产生的IFN-γ呈正相关（P < 0.05）。然而，一些特应性患者在IL-12和IL-18刺激下的IFN-γ产生水平之间存在差异。
此处所示结果提示特应性患者存在IL-12和/或IL-18信号通路异常，如基因缺陷。
