Parthuisot N, Catala P, Lemarchand K, Baudart J, Lebaron P
Observatoire Océanologique, Centre National de la Recherche Scientifique, Institut National des Sciences de l'Univers et Université Pierre et Marie Curie, Banyuls-sur-Mer, France.
J Appl Microbiol. 2000 Aug;89(2):370-80. doi: 10.1046/j.1365-2672.2000.01126.x.
The efficiency of ChemChrome B (CB) and ChemChrome V6 (CV6) dyes to stain viable bacterial cells in water was compared. Both dyes are fluorogenic esters converted to free fluorescein by esterase activity. The dyes were applied to a wide variety of bacterial species, including those poorly stained by CB, and to natural waters. Some species tested gave unacceptable low fluorescence intensities by being inefficiently or non-labelled with the CB. In contrast, CV6-stained bacteria were easily detected by both flow cytometry and solid-phase cytometry. As a consequence, higher viable cell counts were found with CV6 compared with CB in natural waters. Viable counts determined by CV6 staining were always higher than cfu counts. In contrast, respiring cell counts (CTC) were always lower than CV6 counts and, in the case of tap and mineral waters, they were lower than cfu counts.
比较了化学铬B（CB）和化学铬V6（CV6）染料对水中活细菌细胞的染色效率。这两种染料都是荧光酯，可通过酯酶活性转化为游离荧光素。将这些染料应用于多种细菌物种，包括那些用CB染色效果不佳的物种，以及天然水体。一些测试的物种由于被CB标记效率低下或未被标记，导致荧光强度低得不可接受。相比之下，通过流式细胞术和固相细胞术都能轻松检测到用CV6染色的细菌。因此，在天然水体中，与CB相比，CV6检测到的活细胞数更高。通过CV6染色确定的活细胞数总是高于菌落形成单位（cfu）数。相比之下，呼吸细胞数（CTC）总是低于CV6计数，对于自来水和矿泉水，CTC低于cfu计数。
