Rosmond R, Ukkola O, Chagnon M, Bouchard C, Björntorp P
Department of Heart and Lung Diseases, Göteborg University, Göteborg, Sweden.
J Intern Med. 2000 Sep;248(3):239-44. doi: 10.1046/j.1365-2796.2000.00721.x.
To investigate the effect of polymorphisms in codon 16 (Arg16Gly) and codon 27 (Gln27Glu) of the beta2-adrenergic receptor gene (ADRB2) on anthropometric, endocrine, metabolic and haemodynamic variables.
A cross-sectional study.
A subgroup of 284 Swedish men from a population sample of 1040 at the age of 51 years.
Genotype examination of ADRB2 polymorphisms in codon 16 and codon 27 with polymerase chain reaction and restriction fragment length polymorphism. Anthropometric measurements included body mass index, waist-to-hip ratio and abdominal sagittal diameter. Endocrine measurements included blood levels of testosterone, insulin-like growth factor I, and leptin plus salivary cortisol. Overnight fasting values of serum insulin, blood glucose, triglycerides, total, low and high density lipoprotein cholesterol, as well as blood pressure and resting heart rate, were also determined.
Polymorphisms were frequent in both codon 16 and codon 27. The Arg16Gly genotype showed significant relationships to elevated central distribution of body fat and to systolic blood pressure, whilst the Glu27Glu genotype was associated with elevated leptin and triglyceride levels but not to other measurements, including obesity variables.
We conclude that only a few cardiovascular risk factors are associated with DNA sequence variation in the ADRB2 in Swedish men.
研究β2 - 肾上腺素能受体基因（ADRB2）第16密码子（Arg16Gly）和第27密码子（Gln27Glu）的多态性对人体测量学、内分泌、代谢和血流动力学变量的影响。
横断面研究。
从1040名51岁人群样本中选取的284名瑞典男性亚组。
采用聚合酶链反应和限制性片段长度多态性技术对ADRB2基因第16和第27密码子的多态性进行基因型检测。人体测量指标包括体重指数、腰臀比和腹部矢状径。内分泌指标包括睾酮、胰岛素样生长因子I、瘦素以及唾液皮质醇的血浓度。还测定了血清胰岛素、血糖、甘油三酯、总胆固醇、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇的空腹值，以及血压和静息心率。
第16和第27密码子的多态性均较为常见。Arg16Gly基因型与体脂中心分布增加及收缩压显著相关，而Glu27Glu基因型与瘦素和甘油三酯水平升高相关，但与包括肥胖变量在内的其他测量指标无关。
我们得出结论，在瑞典男性中，只有少数心血管危险因素与ADRB2的DNA序列变异有关。
