Macho-Azcarate T, Marti A, González A, Martinez J A, Ibañez J
Department of Physiology and Nutrition, University of Navarra, Pamplona, Spain.
Int J Obes Relat Metab Disord. 2002 Nov;26(11):1434-41. doi: 10.1038/sj.ijo.0802129.
The Glu27Glu genotype in the beta-2-adrenergic receptor (ADRB2) is associated with fat mass, body mass index and obesity in females. In our population, we previously found an association of higher body mass index (BMI) among women who reported more physical activity and carried the Glu27 allele as compared to non carriers with the same level of activity.
To examine the lipid metabolism differences, both at rest and during submaximal exercise in ADRB2 Glu27Glu vs Gln27Gln obese women.
Eight obese women with the Glu27Glu genotype (age, 43+/-5 y; body mass index (BMI), 31.7+/-0.9 kg/m(2); percentage fat mass, 42.0+/-1.3; WHR, 0.83+/-0.02; and VO(2max), 21.6+/-0.9 ml/kg/min) were compared with seven obese women with the Gln27Gln genotype (age, 43+/-5 y; BMI, 33.9+/-1.3 kg/m(2); percentage fat mass, 41.6+/-1.2; WHR, 0.83+/-0.02; and VO(2max), 20.6+/-0.8 ml/kg/min).
The ADRB2 polymorphism was identified by PCR-RFLP. Respiratory quotient was determined by indirect calorimetry at baseline, during 1 h of walking on a treadmill and 1 h after the exercise. Plasma triglycerides, glycerol, FFA, hydroxybutyrate, glucose and lactate were assayed by spectrophotometric methods. Insulin, leptin and progesterone were measured by radioimmunoassay. Adrenaline and noradrenaline were quantified by high performance liquid chromatography.
The ADRB2 Glu27Glu subjects had lower plasma glycerol (P=0.047) and lower hydroxybutyrate (P=0.001) throughout the study than the Gln27Gln group. Plasma triglycerides (P=0.001), lactate (P<0.05) and serum insulin (P<0.05) remained higher in the Glu27Glu group vs the Gln27Gln group. The respiratory quotient (RQ) was higher in the Glu27Glu obese women along the study (P=0.046), and fat oxidation was significantly lower in this group during the recovery (P=0.048). The other variables did not differ statistically between groups.
These data suggest that both lipolysis and fat oxidation promoted by an acute submaximal exercise intervention could be blunted in the polymorphic ADRB2 Glu27Glu group of our female obese population.
β-2肾上腺素能受体（ADRB2）中的Glu27Glu基因型与女性的脂肪量、体重指数和肥胖有关。在我们的人群中，我们之前发现，与相同活动水平的非携带者相比，报告有更多身体活动且携带Glu27等位基因的女性体重指数（BMI）更高。
研究ADRB2 Glu27Glu型与Gln27Gln型肥胖女性在静息状态和次极量运动期间的脂质代谢差异。
8名Glu27Glu基因型肥胖女性（年龄43±5岁；体重指数（BMI）31.7±0.9kg/m²；脂肪量百分比42.0±1.3；腰臀比0.83±0.02；最大摄氧量（VO₂max）21.6±0.9ml/kg/min）与7名Gln27Gln基因型肥胖女性（年龄43±5岁；BMI 33.9±1.3kg/m²；脂肪量百分比41.6±1.2；腰臀比0.83±0.02；最大摄氧量（VO₂max）20.6±0.8ml/kg/min）进行比较。
通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）鉴定ADRB2基因多态性。在基线、跑步机上步行1小时期间及运动后1小时，通过间接测热法测定呼吸商。采用分光光度法测定血浆甘油三酯、甘油、游离脂肪酸（FFA）、羟丁酸、葡萄糖和乳酸。采用放射免疫分析法测定胰岛素、瘦素和孕酮。通过高效液相色谱法定量肾上腺素和去甲肾上腺素。
在整个研究过程中，ADRB2 Glu27Glu受试者的血浆甘油水平（P=0.047）和羟丁酸水平（P=0.001）均低于Gln27Gln组。与Gln27Gln组相比，Glu27Glu组的血浆甘油三酯水平（P=0.001）、乳酸水平（P<0.05）和血清胰岛素水平（P<0.05）仍然更高。在整个研究过程中，Glu27Glu肥胖女性的呼吸商（RQ）更高（P=0.046），且该组在恢复期间的脂肪氧化显著更低（P=0.048）。其他变量在两组之间无统计学差异。
这些数据表明，在我们的女性肥胖人群中，多态性ADRB2 Glu27Glu组中，急性次极量运动干预所促进的脂肪分解和脂肪氧化可能会受到抑制。
