Sáez F J, Madrid J F, Aparicio R, Leis O, Oporto B
Department of Cell Biology and Morphological Sciences, University of the Basque Country, School of Medicine and Dentistry, Leioa (Vizcaya), Spain.
Glycoconj J. 1999 Oct;16(10):639-48. doi: 10.1023/a:1007081101470.
The aim of this work is the characterization of the glycoconjugates of the spermatids during the spermiogenesis of the testis of an urodele amphibian, Pleurodeles waltl, by means of lectins in combination with several chemical and enzymatic procedures, in order to establish the distribution of N- and O-linked oligosaccharides in these cells. The acrosome was the most relevant lectin-labeled structure. The O-linked oligosaccharides contained DBA- and SBA-positive GalNAc, AAA-positive Fuc and PNA-positive Gal beta1,3GalNAc. Sialic acid was scarcely observed, the Neu5Ac alpha2,3Gal beta1,4GlcNAc sequence was found in N-linked oligosaccharides. Additionally, N-linked oligosaccharides containing HPA-positive GalNAc and AAA-positive Fuc were found. Moreover, with some lectins the acrosome showed a variable composition of the oligosaccharides in the different steps of the sperm maturation. Some residues were found only in the early steps in maturating acrosome, while others were in the later steps, showing that acrosomal glycoconjugates are modified during acrosome development in spermiogenesis. The changes observed during acrosome maturation suggest the existence of a predetermined pattern of storage of the acrosome components and a progressive compression of them.
本研究旨在通过凝集素结合多种化学和酶促方法，对有尾两栖动物疣螈精巢精子发生过程中精子细胞的糖缀合物进行表征，以确定这些细胞中N-连接和O-连接寡糖的分布。顶体是凝集素标记最显著的结构。O-连接寡糖含有DBA和SBA阳性的GalNAc、AAA阳性的Fuc以及PNA阳性的Galβ1,3GalNAc。几乎未观察到唾液酸，在N-连接寡糖中发现了Neu5Acα2,3Galβ1,4GlcNAc序列。此外，还发现了含有HPA阳性GalNAc和AAA阳性Fuc的N-连接寡糖。此外，在精子成熟的不同阶段，顶体与某些凝集素结合显示出寡糖组成的变化。一些残基仅在顶体成熟的早期阶段出现，而其他残基则在后期阶段出现，这表明在精子发生过程中顶体发育期间顶体糖缀合物会发生修饰。在顶体成熟过程中观察到的变化表明存在顶体成分的预定储存模式以及它们的逐步压缩。
