Desantis S, Ventriglia G, Zubani D, Deflorio M, Megalofonou P, Acone F, Zarrilli A, Palmieri G, De Metrio G
Department of Animal Health and Well-being, Faculty of Veterinary Medicine, University of Bari, Italy.
Histol Histopathol. 2006 Jan;21(1):11-22. doi: 10.14670/HH-21.11.
The localization and characterization of oligosaccharide sequences in the cat testis was investigated using 12 lectins in combination with the beta-elimination reaction, N-Glycosidase F and sialidase digestion. Leydig cells expressed O-linked glycans with terminal alphaGalNAc (HPA reactivity) and N-glycans with terminal/internal alphaMan (Con A affinity). The basement membrane showed terminal Neu5Acalpha2,6Gal/GalNAc, Galbeta1,3GalNAc, alpha/betaGalNAc, and GlcNAc (SNA, PNA, HPA, SBA, GSA II reactivity) in O-linked oligosaccharides, terminal Galbeta1,4GlcNAc (RCA120 staining) and alphaMan in N-linked oligosaccharides; in addition, terminal Neu5acalpha2,3Galbeta1,4GlcNac, Forssman pentasaccharide, alphaGal, alphaL-Fuc and internal GlcNAc (MAL II, DBA, GSA I-B4, UEA I, KOH-sialidase-WGA affinity) formed both O- and N-linked oligosaccharides. The Sertoli cells cytoplasm contained terminal Neu5Ac-Galbeta1,4GlcNAc, Neu5Ac-betaGalNAc as well as internal GlcNAc in O-linked glycans, alphaMan in N-linked glycoproteins and terminal Neu5Acalpha2,6Gal/ GalNAc in both O- and N-linked oligosaccharides. Spermatogonia exhibited cytoplasmic N-linked glycoproteins with alphaMan residues. The spermatocytes cytoplasm expressed terminal Neu5Acalpha2,3Galbeta1,4 GlcNAc and Galbeta1,3GalNAc in O-linked oligosaccharides, terminal Galbeta1,4GlcNAc and alpha/betaGalNAc in N-linked glycoconjugates. The Golgi region showed terminal Neu5Acalpha2,3Galbeta1,4GlcNac, Galbeta1,4GlcNAc, Forssman pentasaccharide, and alphaGalNAc in O-linked oligosaccharides, alphaMan and terminal betaGal in N-linked oligosaccharides. The acrosomes of Golgi-phase spermatids expressed terminal Galbeta1,3GalNAc, Galbeta1,4GlcNAc, Forssmann pentasaccharide, alpha/betaGalNAc, alphaGal and internal GlcNAc in O-linked oligosaccharides, terminal alpha/betaGalNAc, alphaGal and terminal/internal alphaMan in N-linked glycoproteins. The acrosomes of cap-phase spermatids lacked internal Forssman pentasaccharide and alphaGal, while having increased alpha/betaGalNAc. The acrosomes of elongated spermatids did not show terminal Galbeta1,3GalNAc, displayed terminal Galbeta1,4GlcNAc and alpha/betaGalNAc in N-glycans and Neu5Ac-Galbeta1,3GalNAc in O-linked oligosaccharides.
利用12种凝集素结合β-消除反应、N-糖苷酶F和唾液酸酶消化，对猫睾丸中寡糖序列的定位和特征进行了研究。睾丸间质细胞表达带有末端αGalNAc的O-连接聚糖（对花生凝集素HPA有反应）和带有末端/内部αMan的N-连接聚糖（对刀豆球蛋白A有亲和力）。基底膜在O-连接寡糖中显示末端Neu5Acalpha2,6Gal/GalNAc、Galbeta1,3GalNAc、α/βGalNAc和GlcNAc（对SNA、PNA、HPA、SBA、GSA II有反应），在N-连接寡糖中显示末端Galbeta1,4GlcNAc（RCA120染色）和αMan；此外，末端Neu5acalpha2,3Galbeta1,4GlcNac、福斯曼五糖、αGal、αL-岩藻糖和内部GlcNAc（对MAL II, DBA, GSA I-B4, UEA I, KOH-唾液酸酶-WGA有亲和力）形成O-连接和N-连接寡糖。支持细胞的细胞质在O-连接聚糖中含有末端Neu5Ac-Galbeta1,4GlcNAc、Neu5Ac-βGalNAc以及内部GlcNAc，在N-连接糖蛋白中含有αMan，在O-连接和N-连接寡糖中含有末端Neu5Acalpha2,6Gal/GalNAc。精原细胞表现出带有αMan残基的细胞质N-连接糖蛋白。精母细胞的细胞质在O-连接寡糖中表达末端Neu5Acalpha2,3Galbeta1,4 GlcNAc和Galbeta1,3GalNAc，在N-连接糖缀合物中表达末端Galbeta1,4GlcNAc和α/βGalNAc。高尔基体区域在O-连接寡糖中显示末端Neu5Acalpha2,3Galbeta1,4GlcNac、Galbeta1,4GlcNAc、福斯曼五糖和αGalNAc，在N-连接寡糖中显示αMan和末端βGal。高尔基期精子细胞的顶体在O-连接寡糖中表达末端Galbeta1,3GalNAc、Galbeta1,4GlcNAc、福斯曼五糖、α/βGalNAc、αGal和内部GlcNAc，在N-连接糖蛋白中表达末端α/βGalNAc、αGal和末端/内部αMan。帽期精子细胞的顶体缺乏内部福斯曼五糖和αGal，但α/βGalNAc增加。伸长型精子细胞的顶体不显示末端Galbeta1,3GalNAc，在N-聚糖中显示末端Galbeta1,4GlcNAc和α/βGalNAc，在O-连接寡糖中显示Neu5Ac-Galbeta1,3GalNAc。
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