Vantarakis A, Komninou G, Venieri D, Papapetropoulou M
Environmental Microbiology, Lab of Public Health, Medical School, University of Patras, Greece.
Lett Appl Microbiol. 2000 Aug;31(2):105-9. doi: 10.1046/j.1365-2672.2000.00797.x.
Multiplex PCR amplification of invA and virA genes was developed enabling simultaneous detection in mussels of Salmonella spp. and Shigella spp., respectively. Simultaneous amplification of products of 215 and 275 bp was obtained either by using mixtures of individual strains of Sh. dysenteriae and Salm. typhimurium or spiked contaminated mussels with both bacteria. In the case of the mussels, 10-100 cells of Salmonella spp. and Shigella per millilitre of homogenate were detected by the multiplex PCR following a pre-enrichment step to increase sensitivity and to ensure that detection was based on the presence of cultivable bacteria. Also, the sensitivity and specificity of this method was evaluated. Multiplex PCR amplification was shown to be an effective, sensitive and rapid method for the simultaneous detection of pathogens in mussels.
开发了invA和virA基因的多重PCR扩增方法，能够分别同时检测贻贝中的沙门氏菌属和志贺氏菌属。通过使用痢疾志贺氏菌和鼠伤寒沙门氏菌的单个菌株混合物或用两种细菌加标的受污染贻贝，可同时获得215和275 bp产物的扩增。对于贻贝，在进行预富集步骤以提高灵敏度并确保检测基于可培养细菌的存在之后，通过多重PCR检测到每毫升匀浆中有10 - 100个沙门氏菌属细胞和志贺氏菌。此外，还评估了该方法的灵敏度和特异性。多重PCR扩增被证明是一种用于同时检测贻贝中病原体的有效、灵敏且快速的方法。
