用于检测蛋黄酱中志贺氏菌属的聚合酶链反应
PCR for detection of Shigella spp. in mayonnaise.
作者信息
Villalobo E, Torres A
机构信息
Departamento Microbiología, Facultad Biología, Universidad Sevilla, Spain.
出版信息
Appl Environ Microbiol. 1998 Apr;64(4):1242-5. doi: 10.1128/AEM.64.4.1242-1245.1998.
Abstract
The use of PCR to amplify a specific virA gene fragment serves as a highly specific and sensitive method to detect virulent bacteria of the genus Shigella and enteroinvasive Escherichia coli. Amplification of a 215-bp DNA band was obtained by using isolated genomic DNA of Shigella, individual cells of Shigella dysenteriae, and mayonnaise contaminated with S. dysenteriae. Moreover, a multiplex PCR with specific (virA) and bacterium-restricted (16S ribosomal DNA) primers generated an amplification product of approximately 755 bp for all bacteria tested and an additional 215-bp product for Shigella and enteroinvasive E. coli.
摘要
使用聚合酶链反应（PCR）扩增特定的virA基因片段，是检测志贺氏菌属和肠侵袭性大肠杆菌等致病细菌的一种高度特异且灵敏的方法。通过使用志贺氏菌的分离基因组DNA、痢疾志贺氏菌的单个细胞以及被痢疾志贺氏菌污染的蛋黄酱，获得了一条215碱基对（bp）的DNA条带扩增产物。此外，使用特异性（virA）引物和细菌限制性（16S核糖体DNA）引物进行的多重PCR，对所有测试细菌产生了一条约755 bp的扩增产物，对志贺氏菌和肠侵袭性大肠杆菌还产生了一条额外的215 bp产物。
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