Arana I, Santorum P, Muela A, Barcina I
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Ciencias, Universidad del País Vasco, Bilbao, Spain.
Lett Appl Microbiol. 2000 Aug;31(2):157-62. doi: 10.1046/j.1365-2672.2000.00793.x.
Quantitative and qualitative changes in organic matter content of wastewater effluents attributable to chlorination and ozonation have been analysed using bioassays as well as organic carbon direct measures. Bioassays were carried out using the bacterial populations of wastewater and two Escherichia coli strains as test micro-organisms. Our results indicate that pure strains present some advantages over indigenous bacteria. Although wastewater bacterial populations are better adapted to growth in wastewater, E. coli strains are more sensitive to changes in dissolved organic carbon (DOC) content. Moreover, the use of pure cultures allows estimation of the portion of DOC which can be converted in cell biomass, the assimilable organic carbon (AOC). Finally, the results obtained using prototrophic and the auxotrophic strains of E. coli suggested that ozonation alters the amino acid composition of wastewater while chlorination does not change the quantity nor the quality of the DOC present in effluents.
利用生物测定法以及有机碳直接测量法，分析了氯化和臭氧化处理导致的废水流出物中有机物含量的定量和定性变化。生物测定使用废水细菌种群以及两种大肠杆菌菌株作为测试微生物。我们的结果表明，纯菌株相对于本地细菌具有一些优势。尽管废水细菌种群更适应在废水中生长，但大肠杆菌菌株对溶解有机碳（DOC）含量的变化更敏感。此外，使用纯培养物可以估算出可转化为细胞生物质的DOC部分，即可同化有机碳（AOC）。最后，使用大肠杆菌的原养型和营养缺陷型菌株获得的结果表明，臭氧化会改变废水的氨基酸组成，而氯化不会改变流出物中DOC的数量和质量。
