Arana I, Santorum P, Muela A, Barcina I
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Ciencias, Universidad del País Vasco, Bilbao, Spain.
J Appl Microbiol. 1999 May;86(5):883-8. doi: 10.1046/j.1365-2672.1999.00772.x.
The effect of chlorine and ozone on Escherichia coli cells resuspended in waste-water was compared. Selected chlorination and ozonation conditions produced a similar decrease in culturability (2-2.5 log). Under these conditions, differences in membrane permeability and cell surface hydrophobicity, depending on the disinfectant tested, were detected. After ozonation, while no changes in cell surface hydrophobicity were observed, approximately 95.5% of cells showed altered membrane permeability. The effect of chlorine was not linked to changes in membrane permeability. After chlorination, E. coli cells showed a tendancy to aggregate. The possibility that aggregation of cells could interfere with conventional colony counts is discussed. The degree of toxicity (Microtox assay) was unrelated to the effect on cellular activity.
比较了氯和臭氧对废水中重悬的大肠杆菌细胞的影响。选定的氯化和臭氧化条件导致可培养性出现类似程度的降低（2 - 2.5个对数级）。在这些条件下，根据所测试的消毒剂不同，检测到膜通透性和细胞表面疏水性存在差异。臭氧化后，虽然未观察到细胞表面疏水性的变化，但约95.5%的细胞显示出膜通透性改变。氯的作用与膜通透性的变化无关。氯化后，大肠杆菌细胞呈现聚集趋势。讨论了细胞聚集可能干扰传统菌落计数的可能性。毒性程度（微毒性测定）与对细胞活性的影响无关。
