Zschöck M, Hamann H P, Kloppert B, Wolter W
Staatliches Medizinal, Lebensmittel und Veterinäruntersuchungsamt, Mittelhessen, Giessen, Germany.
Lett Appl Microbiol. 2000 Sep;31(3):203-8. doi: 10.1046/j.1365-2672.2000.00789.x.
The prevalence of Shiga-toxin-producing Escherichia coli (STEC) in healthy dairy ruminants was investigated between 1996 and 1998 by a multiplex polymerase chain reaction (mPCR) technique. A total of 13 552 E. coli colonies from 726 cows, 28 sheep and 93 goats out of 112 randomly selected dairy farms in Hessia, Germany were analysed. STEC strains were recovered from 131 (18.0%) cows, nine (32.1%) sheep and 70 (75.3%) goats. Further characterization of the STEC isolates showed that 89 (0.66% of the investigated colonies) of animal field strains carried stx1 gene, 64 (0.47%) stx2 gene and 57 (0.42%) stx1 and stx2 gene. Sixty (93.8%) out of 64 stx2 field strains were harboured by cows. In contrast, 74 (83.1%) out of 89 stx1 dairy animal field strains were from ovine or caprine origin. Only 17 (8. 1%) stx-positive isolates (13 from cattle, three from sheep and only one from goat) were positive for eaeA gene. Eight (9.0%) of the stx1, five (7.8%) of the stx2 and four (7.0%) of the stx1/stx2 gene-positive field strains carried the eaeA gene. The prevalence of EHEC-haemolysin (EHEC-hlyA) gene sequence was 88.8% (79 isolates) of the stx1 and 68.8% (44 isolates) of the stx2 isolates. Out of 57 stx1- and stx2-positive field-strains, 34 (59.6%) carried the EHEC-hlyA gene. E. coli O serovars O:157 and O:111 were not found. Only one isolate was positive with O26 antiserum.
1996年至1998年期间，采用多重聚合酶链反应（mPCR）技术对健康奶牛反刍动物中产志贺毒素大肠杆菌（STEC）的流行情况进行了调查。对德国黑森州112个随机选取的奶牛场中的726头奶牛、28只绵羊和93只山羊的总共13552个大肠杆菌菌落进行了分析。从131头（18.0%）奶牛、9只（32.1%）绵羊和70只（75.3%）山羊中分离出了STEC菌株。对STEC分离株的进一步鉴定表明，89株（占所调查菌落的0.66%）动物田间菌株携带stx1基因，64株（0.47%）携带stx2基因，57株（0.42%）同时携带stx1和stx2基因。64株stx2田间菌株中有60株（93.8%）来自奶牛。相比之下，89株stx1奶牛动物田间菌株中有74株（83.1%）来自绵羊或山羊。只有17株（8.1%）stx阳性分离株（13株来自牛，3株来自绵羊，仅1株来自山羊）eaeA基因呈阳性。stx1基因阳性田间菌株中有八株（9.0%）、stx2基因阳性田间菌株中有五株（7.8%）、stx1/stx2基因阳性田间菌株中有四株（7.0%）携带eaeA基因。EHEC溶血素（EHEC-hlyA）基因序列的流行率在stx1分离株中为88.8%（79株），在stx2分离株中为68.8%（44株）。在57株stx1和stx2阳性田间菌株中，34株（59.6%）携带EHEC-hlyA基因。未发现大肠杆菌O血清型O:157和O:111。只有一株分离株与O26抗血清呈阳性反应。
