Datwyler S A, Meares C F
Dept of Chemistry, University of California, One Shields Avenue, Davis, CA 95616-5295, USA.
Trends Biochem Sci. 2000 Sep;25(9):408-14. doi: 10.1016/s0968-0004(00)01652-2.
Interactions between proteins are important to understand but difficult to study. Conjugating a protein to a small artificial protease endows it with the ability to cut other proteins where it binds to them. Analysing the sites cut on the target proteins leads to new understanding of the structure of each complex. The binding of sigma factors to a common region on RNA polymerase provides an example.
蛋白质之间的相互作用对于理解至关重要，但却难以研究。将一种蛋白质与一种小型人工蛋白酶结合，能使其具备在与其他蛋白质结合的部位切割这些蛋白质的能力。分析目标蛋白质上被切割的位点有助于对每个复合物的结构有新的认识。例如，西格玛因子与RNA聚合酶上的一个共同区域的结合。
