金黄色葡萄球菌中α-溶血素的细胞定位

Cellular location of alpha-hemolysin in Staphylococcus aureus.

作者信息

Nugent K M, Calandra G B, Theodore T S

出版信息

Infect Immun. 1975 Aug;12(2):270-3. doi: 10.1128/iai.12.2.270-273.1975.

DOI:10.1128/iai.12.2.270-273.1975

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC415280/

Abstract

Protoplast fractionation techniques were used to prepare subcellular fractions of three strains of Staphylococcus aureus for the analysis of alpha-hemolysin activity. More than 90% of the cellular hemolytic activity was localized in the periplasm, only a trace was detected in the cytoplasm, and none was found in either plasma membranes or mesosomes. This cellular portion constituted approximately 4 to 10% of the total hemolytic activity of the culture. The erythrocyte lysis spectrum and Sephadex G-100 filtration studies indicated that the cellular hemolysin closely resembles the extracellular form.

摘要

采用原生质体分级分离技术制备了三株金黄色葡萄球菌的亚细胞级分，用于分析α-溶血素活性。超过90%的细胞溶血活性定位于周质，仅在细胞质中检测到微量，而在质膜或间体中均未发现。该细胞级分约占培养物总溶血活性的4%至10%。红细胞裂解谱和葡聚糖G-100过滤研究表明，细胞溶血素与细胞外形式非常相似。

相似文献

1

Cellular location of alpha-hemolysin in Staphylococcus aureus.金黄色葡萄球菌中α-溶血素的细胞定位

Infect Immun. 1975 Aug;12(2):270-3. doi: 10.1128/iai.12.2.270-273.1975.

2

Quantitative Hemolysis Assays.定量溶血检测。

Methods Mol Biol. 2021;2341:25-30. doi: 10.1007/978-1-0716-1550-8_4.

3

Biological properties of extracellular proteins from staphylococcus.葡萄球菌细胞外蛋白质的生物学特性

Ann N Y Acad Sci. 1974 Jul 31;236(0):343-61. doi: 10.1111/j.1749-6632.1974.tb41502.x.

4

[Gamma hemolysin of Staphylococcus aureus: purification and properties].[金黄色葡萄球菌的γ溶血素：纯化及特性]

Ann Inst Pasteur (Paris). 1970 Jan;118(1):19-33.

5

Cell-associated alpha-toxin from Staphylococcus aureus.金黄色葡萄球菌的细胞相关α毒素

J Med Microbiol. 1972 Feb;5(1):123-7. doi: 10.1099/00222615-5-1-123.

6

Restriction fragment length polymorphisms associated with alpha-hemolysin determinants are correlating with the expression of alpha-hemolysin in strains of Escherichia coli.与α-溶血素决定簇相关的限制性片段长度多态性与大肠杆菌菌株中α-溶血素的表达相关。

Zentralbl Bakteriol. 1992 Jan;276(2):152-64. doi: 10.1016/s0934-8840(11)80002-2.

7

TYPES OF HEMOLYSINS PRODUCED BY STAPHYLOCOCCUS AUREUS, AS DETERMINED BY THE REPLICA PLATING TECHNIQUE.通过影印平板技术确定的金黄色葡萄球菌产生的溶血素类型

J Bacteriol. 1964 Nov;88(5):1442-7. doi: 10.1128/jb.88.5.1442-1447.1964.

8

Functional expression of the alpha-hemolysin of Staphylococcus aureus in intact Escherichia coli and in cell lysates. Deletion of five C-terminal amino acids selectively impairs hemolytic activity.金黄色葡萄球菌α-溶血素在完整大肠杆菌及细胞裂解物中的功能性表达。C末端五个氨基酸的缺失选择性地损害溶血活性。

J Biol Chem. 1992 May 25;267(15):10902-9.

9

Comparison of purified alpha-toxins from various strains of Staphylococcus aureus.来自不同金黄色葡萄球菌菌株的纯化α毒素的比较。

Appl Microbiol. 1974 Jul;28(1):86-90. doi: 10.1128/am.28.1.86-90.1974.

10

Further characterization of staphylococcal gamma-hemolysin.葡萄球菌γ-溶血素的进一步特性分析。

Infect Immun. 1974 Jul;10(1):54-9. doi: 10.1128/iai.10.1.54-59.1974.

本文引用的文献

1

Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。

J Biol Chem. 1951 Nov;193(1):265-75.

2

Isolation and composition of staphylococcal alpha toxin.葡萄球菌α毒素的分离与组成

J Gen Microbiol. 1963 Mar;30:455-68. doi: 10.1099/00221287-30-3-455.

3

A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。

Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.

4

An intracellular hemolysin of group A Streptococci. I. influence of sonic energy and pH on hemolytic potency.A组链球菌的一种细胞内溶血素。I. 声能和pH对溶血效力的影响。

J Bacteriol. 1956 Jan;71(1):94-9. doi: 10.1128/jb.71.1.94-99.1956.

5

The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。

J Biol Chem. 1969 Aug 25;244(16):4406-12.

6

Ultracentrifugal analysis of staphylococcal alpha toxin.葡萄球菌α毒素的超速离心分析

J Bacteriol. 1971 Apr;106(1):138-42. doi: 10.1128/jb.106.1.138-142.1971.

7

Production of staphylococcal alpha toxin. I. Relationship between cell growth and toxin formation.葡萄球菌α毒素的产生。I. 细胞生长与毒素形成之间的关系。

Infect Immun. 1971 Oct;4(4):456-61. doi: 10.1128/iai.4.4.456-461.1971.

8

The separation and isolation of plasma membranes and mesosomal vesicles from Staphylococcus aureus.从金黄色葡萄球菌中分离和提取质膜和间体囊泡。

Prep Biochem. 1971;1(3):233-48. doi: 10.1080/00327487108081942.

9

Cell-associated alpha-toxin from Staphylococcus aureus.金黄色葡萄球菌的细胞相关α毒素

J Med Microbiol. 1972 Feb;5(1):123-7. doi: 10.1099/00222615-5-1-123.

10

Physical states of staphylococcal alpha-toxin.葡萄球菌α毒素的物理状态

J Bacteriol. 1967 Oct;94(4):1170-7. doi: 10.1128/jb.94.4.1170-1177.1967.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。