Nugent K M, Calandra G B, Theodore T S
Infect Immun. 1975 Aug;12(2):270-3. doi: 10.1128/iai.12.2.270-273.1975.
Protoplast fractionation techniques were used to prepare subcellular fractions of three strains of Staphylococcus aureus for the analysis of alpha-hemolysin activity. More than 90% of the cellular hemolytic activity was localized in the periplasm, only a trace was detected in the cytoplasm, and none was found in either plasma membranes or mesosomes. This cellular portion constituted approximately 4 to 10% of the total hemolytic activity of the culture. The erythrocyte lysis spectrum and Sephadex G-100 filtration studies indicated that the cellular hemolysin closely resembles the extracellular form.
采用原生质体分级分离技术制备了三株金黄色葡萄球菌的亚细胞级分，用于分析α-溶血素活性。超过90%的细胞溶血活性定位于周质，仅在细胞质中检测到微量，而在质膜或间体中均未发现。该细胞级分约占培养物总溶血活性的4%至10%。红细胞裂解谱和葡聚糖G-100过滤研究表明，细胞溶血素与细胞外形式非常相似。
