葡萄球菌γ-溶血素的进一步特性分析。
Further characterization of staphylococcal gamma-hemolysin.
作者信息
Taylor A G, Bernheimer A W
出版信息
Infect Immun. 1974 Jul;10(1):54-9. doi: 10.1128/iai.10.1.54-59.1974.
Abstract
It was confirmed that staphylococcal gamma-hemolysin is composed of two separate proteins (gamma-lysin components I and II) which act synergistically. The molecular weights of the two components, determined by gel filtration, are 29,000 and 26,000, respectively, and their isoelectric points, determined by isoelectric focusing, are at pH 9.8 and 9.9. Both components are susceptible to the action of Pronase and subtilisin. A wide range of lipids, some in minute amounts, are capable of inhibiting the hemolytic activity of gamma-lysin.
摘要
已证实葡萄球菌γ-溶血素由两种单独的蛋白质（γ-溶血素组分I和II）组成，它们协同发挥作用。通过凝胶过滤测定的这两种组分的分子量分别为29,000和26,000，通过等电聚焦测定的它们的等电点分别在pH 9.8和9.9。两种组分都易受链霉蛋白酶和枯草杆菌蛋白酶的作用。多种脂质，有些含量极少，能够抑制γ-溶血素的溶血活性。
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