针对结构蛋白和非结构蛋白的人类登革热抗体。
Human Dengue antibodies against structural and nonstructural proteins.
作者信息
Valdés K, Alvarez M, Pupo M, Vázquez S, Rodríguez R, Guzmán M G
机构信息
Department of Virology, PAHO/WHO Collaborating Center for Viral Diseases, "Pedro Kouri" Tropical Medicine Institute, Autopista Novia del Mediodía, Marianao 13, Havana, Cuba.
出版信息
Clin Diagn Lab Immunol. 2000 Sep;7(5):856-7. doi: 10.1128/CDLI.7.5.856-857.2000.
Abstract
Antibodies against dengue virus type 2 and 4 proteins in acute-phase sera of 10 primary and 10 secondary dengue fever and dengue hemorrhagic fever patients were studied by Western blotting. In the first group the immune response was barely detectable, while in the second group more proteins were detected, with a very strong reaction. Anti-NS1 and -NS3 antibodies were detected mainly in secondary cases. Anti-E, -NS3, and -NS5 antibodies were detected in a high number of cases. The possibility of implementing early diagnostic assays for antigen detection is suggested.
摘要
通过蛋白质印迹法研究了10例初次感染和10例再次感染登革热及登革出血热患者急性期血清中针对2型和4型登革病毒蛋白的抗体。在第一组中，免疫反应几乎检测不到，而在第二组中检测到更多蛋白质，且反应非常强烈。抗NS1和抗NS3抗体主要在再次感染病例中检测到。在大量病例中检测到抗E、抗NS3和抗NS5抗体。这提示了实施抗原检测早期诊断试验的可能性。
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