Morris D C, Davies K, Zhang Z, Chopp M
Henry Ford Health Sciences Center, Department of Emergency Medicine, 2799 West Grand Boulevard, Detroit, MI 48202, USA.
Brain Res. 2000 Sep 8;876(1-2):31-6. doi: 10.1016/s0006-8993(00)02543-9.
Perfusion of the microcirculation after stroke is central to preserving neuronal function and improving functional outcome. Using laser scanning confocal microscopy (LSCM) and a novel computer software program, MIRAGE, we measured a reduction in cerebral microvessel diameter after 1 and 4 h of embolic middle cerebral artery (MCA) occlusion. The methodology used by MIRAGE for measurement of blood vessel diameter involves a linearly applied spherical inflation technique. Three-dimensional quantitative analysis revealed a significant (P<0.05) mean 10-12% reduction in vessel diameter in the ipsilateral cortex when compared to the homologous region in the contralateral hemisphere. This reduction was seen only in the cortex and not the striatum. A larger reduction in mean vessel diameter in the ipsilateral cortex, 16-30% (P<0.05) was observed when compared to sham control rats. These results are in strong agreement with other studies using different stroke models and imaging techniques. Our work represents a novel application of LSCM technology to the three dimensional investigation of microvessel diameter changes in acute stroke and identifies its potential as an important tool for investigation of cerebral pathology.
中风后微循环灌注对于维持神经元功能和改善功能预后至关重要。我们使用激光扫描共聚焦显微镜（LSCM）和一种新型计算机软件程序MIRAGE，测量了栓塞性大脑中动脉（MCA）闭塞1小时和4小时后脑微血管直径的减小。MIRAGE用于测量血管直径的方法涉及线性应用的球形膨胀技术。三维定量分析显示，与对侧半球的同源区域相比，同侧皮质的血管直径平均显著减小（P<0.05）10-12%。这种减小仅在皮质中出现，而在纹状体中未出现。与假手术对照组大鼠相比，同侧皮质的平均血管直径减小幅度更大，为16-30%（P<0.05）。这些结果与使用不同中风模型和成像技术的其他研究结果高度一致。我们的工作代表了LSCM技术在急性中风微血管直径变化三维研究中的新应用，并确定了其作为研究脑病理学重要工具的潜力。
