Morris Daniel C, Yeich Thomas, Khalighi Mohammad Mehdi, Soltanian-Zadeh Hamid, Zhang Zheng G, Chopp Michael
Departments of Emergency Medicine, Henry Ford Health System, 2799 West Grand Blvd., Detroit, MI 48202, USA.
Brain Res. 2003 May 16;972(1-2):31-7. doi: 10.1016/s0006-8993(03)02433-8.
We analyze morphological alterations of cerebral neovascularization after stroke using a new 3D imaging software program.
Male Wistar rats underwent unilateral embolic middle cerebral artery occlusion (MCAo) by a single fibrin rich clot. Subjects were sacrificed from 1 to 28 days post infarct. Vessel perimeters were measured on coronal sections stained with endothelial cell-specific antibody to von Willebrand's factor. Vessel segment lengths, diameters and number of vessels were analyzed on cerebral microvessels perfused with FITC-dextran 14 days after ischemia using LSCM and a 3-D vessel quantification program.
The mean number of microvessels with enlarged perimeters significantly increased in the ipsilateral cortex at day 7 when compared to the contralateral cortex (29.7+/-14.7 vs. 3.7+/-2.5, P<0.05). Subsequently, differences in the number of microvessels with enlarged perimeters decreased on days 14 and 28. Fourteen days post-MCA occlusion, microvessel segment length (15.0 vs. 26.0 microm, P<0.05) and diameter (3.14 vs. 3.75 microm, P<0.05) significantly decreased in the ipsilateral hemisphere when compared to the contralateral hemisphere, respectively. Furthermore, the mean total number of these smaller microvessels increased in the ipsilateral hemisphere (57.33+/-14.5 vs. 32.22+/-11.7, P<0.05).
Focal cerebral ischemia induces morphological changes (early dilated microvessels followed by decreased microvessel segment length and diameter) that are consistent with newly generated microvessels.
我们使用一种新的三维成像软件程序分析中风后脑新生血管形成的形态学改变。
雄性Wistar大鼠通过单个富含纤维蛋白的血凝块进行单侧栓塞大脑中动脉闭塞（MCAo）。在梗死发生后1至28天处死实验对象。用针对血管性血友病因子的内皮细胞特异性抗体对冠状切片进行染色，测量血管周长。在缺血14天后，使用激光扫描共聚焦显微镜（LSCM）和三维血管定量程序分析灌注了异硫氰酸荧光素-葡聚糖（FITC-葡聚糖）的脑微血管的血管段长度、直径和血管数量。
与对侧皮质相比，同侧皮质在第7天时，血管周长增大的微血管平均数量显著增加（29.7±14.7对3.7±2.5，P<0.05）。随后，在第14天和第28天时，血管周长增大的微血管数量差异减小。大脑中动脉闭塞14天后，与对侧半球相比，同侧半球的微血管段长度（15.0对26.0微米，P<0.05）和直径（3.14对3.75微米，P<0.05）分别显著减小。此外，同侧半球中这些较小微血管的平均总数增加（57.33±14.5对32.22±11.7，P<0.05）。
局灶性脑缺血诱导了与新生微血管一致的形态学变化（早期微血管扩张，随后微血管段长度和直径减小）。
