宽吻海豚黄素细胞色素b gp91(phox)和p22(phox)亚基的分子克隆与鉴定

Molecular cloning and identification of bottle-nosed dolphin flavocytochrome b gp91(phox) and p22(phox) subunits.

作者信息

Inoue Y, Itou T, Jimbo T, Sakai T, Ueda K, Imajoh-Ohmi S, Iida T

机构信息

Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan.

出版信息

Vet Immunol Immunopathol. 2000 Aug 31;76(1-2):137-50. doi: 10.1016/s0165-2427(00)00207-5.

DOI:10.1016/s0165-2427(00)00207-5

PMID:10973692

Abstract

The bottle-nosed dolphin (Tursiops truncatus) gp91(phox) and p22(phox) cDNA were cloned from mitogen stimulated leukocytes RNA utilizing the reverse transcription-polymerase chain reaction. The sequences of these cDNAs showed that dolphin gp91(phox) and p22(phox) clones contained open reading frames encoding 569 and 192 amino acids, respectively. Analysis of the gp91(phox) amino acids sequence showed three potential N-linked glycosylation sites. Comparison of the deduced amino acid showed that dolphin gp91(phox) sequence shared 95.4, 93.8, 91.4 and 89.5% similarity with the bovine, porcine, human and mouse gp91(phox) sequences, respectively. Similarly, the amino acid sequence showed that dolphin p22(phox) shared 89.7, 84.6, 84.1, 83.6 and 83.6% similarity with the bovine, mouse, porcine, human and rattus p22(phox) sequences, respectively. Western blotting analysis with anti-peptide antibodies supported the molecular weights of the dolphin gp91(phox) and p22(phox) homologous proteins predicted from the cDNAs and amino acids sequence data.

摘要

利用逆转录-聚合酶链反应从丝裂原刺激的白细胞RNA中克隆出宽吻海豚（瓶鼻海豚，Tursiops truncatus）的gp91(phox)和p22(phox) cDNA。这些cDNA序列显示，海豚gp91(phox)和p22(phox)克隆分别包含编码569和192个氨基酸的开放阅读框。对gp91(phox)氨基酸序列的分析显示有三个潜在的N-连接糖基化位点。推导氨基酸的比较表明，海豚gp91(phox)序列与牛、猪、人和小鼠的gp91(phox)序列的相似性分别为95.4%、93.8%、91.4%和89.5%。同样，氨基酸序列显示，海豚p22(phox)与牛、小鼠、猪、人和大鼠的p22(phox)序列的相似性分别为89.7%、84.6%、84.1%、83.6%和83.6%。用抗肽抗体进行的蛋白质印迹分析支持了根据cDNA和氨基酸序列数据预测的海豚gp91(phox)和p22(phox)同源蛋白的分子量。