宽吻海豚（瓶鼻海豚）白细胞介素-1α和-1β互补DNA的克隆与测序

Cloning and sequencing of a bottle-nosed dolphin (Tursiops truncatus) interleukin-1alpha and -1beta complementary DNAs.

作者信息

Inoue Y, Itou T, Ueda K, Oike T, Sakai T

机构信息

Department of Preventive Veterinary Medicine and Animal Health, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan.

出版信息

J Vet Med Sci. 1999 Dec;61(12):1317-21. doi: 10.1292/jvms.61.1317.

DOI:10.1292/jvms.61.1317

PMID:10651053

Abstract

The bottle-nosed dolphin (Tursiops truncatus) IL-1alpha and IL-1beta cDNA were cloned from mitogen stimulated peripheral blood mononuclear cells (PBMC) RNA utilizing the reverse transcription-polymerase chain reaction (RT-PCR). The sequences of these cDNAs showed that dolphin IL-1alpha and IL-1beta clones contained open reading frames encoding 265 and 266 amino acids, respectively. Comparison of the deduced amino acid showed that dolphin IL-1alpha sequence shared 77, 77, 77, 74, 71, 65 and 57% similarity with the bovine, ovine, porcine, equine, feline, human and mouse IL-1alpha sequences, respectively. Similarly, the amino acid sequence showed that dolphin IL-1beta shared 77, 77, 74, 69, 65, 64 and 63% similarity with the bovine, ovine, porcine, equine, feline, human and mouse IL-1beta sequences, respectively. The relatedness of dolphin IL-1alpha and IL-1beta were relatively distant with 21% amino acid homology.

摘要

利用逆转录-聚合酶链反应（RT-PCR），从有丝分裂原刺激的外周血单核细胞（PBMC）RNA中克隆出瓶鼻海豚（宽吻海豚）的IL-1α和IL-1β cDNA。这些cDNA序列显示，海豚IL-1α和IL-1β克隆分别包含编码265和266个氨基酸的开放阅读框。对推导的氨基酸进行比较表明，海豚IL-1α序列与牛、羊、猪、马、猫、人和小鼠的IL-1α序列的相似性分别为77%、77%、77%、74%、71%、65%和57%。同样，氨基酸序列显示，海豚IL-1β与牛、羊、猪、马、猫、人和小鼠的IL-1β序列的相似性分别为77%、77%、74%、69%、65%、64%和63%。海豚IL-1α和IL-1β的相关性相对较远，氨基酸同源性为21%。