大肠杆菌新的磷酸葡萄糖异构酶突变体。
New phosphoglucose isomerase mutants of Escherichia coli.
作者信息
Vinopal R T, Hillman J D, Schulman H, Reznikoff W S, Fraenkel D G
出版信息
J Bacteriol. 1975 Jun;122(3):1172-4. doi: 10.1128/jb.122.3.1172-1174.1975.
Abstract
The mutants used to show that phosphoglucose isomerase, and glucose itself, are not essential components of Escherichia coli had not been characterized genetically, other than by mapping. We now describe two new pgi mutants, one amber and the other a Mu-phage insertion, presumably both complete inactivation mutations. The new mutations do not give a phenotype markedly different from those described earlier. However, they might be preferred for certain physiological studies, and we have prepared for this a new double mutant, strain DF214, with a Mu insertion in pgi and a deletion in zwf (flucose 6-phosphate dehydrogenase).
摘要
用于证明磷酸葡萄糖异构酶和葡萄糖本身并非大肠杆菌必需成分的突变体，除了通过定位外，尚未进行遗传学特征描述。我们现在描述两个新的pgi突变体，一个是琥珀突变，另一个是Mu噬菌体插入突变，推测两者均为完全失活突变。这些新突变产生的表型与先前描述的并无明显差异。然而，在某些生理学研究中它们可能更受青睐，为此我们构建了一个新的双突变体DF214菌株，其pgi基因中有一个Mu插入，zwf（葡萄糖6-磷酸脱氢酶）基因中有一个缺失。
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本文引用的文献
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