Marcoux C, Hopkins P N, Wang T, Leary E T, Nakajima K, Davignon J, Cohn J S
Hyperlipidemia and Atherosclerosis Research Group, Clinical Research Institute of Montreal, Quebec, Canada H2W 1R7.
J Lipid Res. 2000 Sep;41(9):1428-36.
Potentially atherogenic triglyceride-rich lipoprotein (TRL) remnants can be isolated and quantitated as remnant-like particles (RLP), using an immunoaffinity gel containing specific anti-human apolipoprotein A-I (apoA-I) and apoB-100 monoclonal antibodies. The aim of the present study was to determine the relationship between postprandial changes in RLP levels and changes in total serum triglyceride (TG) in patients with different forms of hypertriglyceridemia (HTG). Three groups of patients were selected, having similarly elevated serum TG levels: a) HTG with TRL remnant accumulation (i.e., type III patients, n = 15, TG: 3.8 +/- 0.2 mm), b) HTG with increased LDL (i.e., type IIb patients, n = 15, TG: 3.7 +/- 0.2 mm), and c) HTG without evidence of remnant or LDL accumulation (i.e., type IV patients, n = 15, TG: 3.9 +/- 0.3 mm). Ingestion of a 45-g fat meal caused a significant increase in serum TG (30;-50%) in all patients. Mean serum TG levels of the three groups were not significantly different at 4 or 6 h after the meal. RLP cholesterol (C) and TG levels increased after the meal in all patients, but these postprandial increases were also not significantly different among groups. Type III patients had significantly higher (P < 0.01) levels of RLP-C and RLP-apoE in the fasted and fed state, and also had significantly higher RLP-C-to-serum TG ratios (P < 0.001) compared with the other groups. These results indicate that 1) RLP-C and RLP-TG levels are significantly increased in the fed versus fasted state in patients with elevated fasting TG levels; 2) patients with different forms of HTG, but similar TG levels, have similar postprandial increases in RLP-C and RLP-TG; and 3) type III patients have significantly elevated levels of RLP-C and RLP-apoE in both the fed and fasted state.
利用含有特异性抗人载脂蛋白A-I（apoA-I）和apoB-100单克隆抗体的免疫亲和凝胶，可分离并定量具有潜在致动脉粥样硬化性的富含甘油三酯脂蛋白（TRL）残余物，将其作为残余样颗粒（RLP）进行测定。本研究的目的是确定不同类型高甘油三酯血症（HTG）患者餐后RLP水平变化与血清总甘油三酯（TG）变化之间的关系。选取了三组血清TG水平同样升高的患者：a）伴有TRL残余物蓄积的HTG（即Ⅲ型患者，n = 15，TG：3.8±0.2 mmol/L），b）低密度脂蛋白（LDL）升高的HTG（即Ⅱb型患者，n = 15，TG：3.7±0.2 mmol/L），以及c）无残余物或LDL蓄积证据的HTG（即Ⅳ型患者，n = 15，TG：3.9±0.3 mmol/L）。摄入45 g脂肪餐导致所有患者血清TG显著升高（30% - 50%）。餐后4小时或6小时，三组患者的平均血清TG水平无显著差异。所有患者餐后RLP胆固醇（C）和TG水平均升高，但餐后这些升高在组间也无显著差异。Ⅲ型患者在空腹和进食状态下的RLP-C和RLP-apoE水平均显著更高（P < 0.01），与其他组相比，其RLP-C与血清TG的比值也显著更高（P < 0.001）。这些结果表明：1）空腹TG水平升高的患者，进食状态下的RLP-C和RLP-TG水平较空腹状态显著升高；2）不同类型的HTG患者，尽管TG水平相似，但餐后RLP-C和RLP-TG的升高相似；3）Ⅲ型患者在进食和空腹状态下的RLP-C和RLP-apoE水平均显著升高。
