Brocklehurst Kathryn R, Morby Andrew P
School of Biosciences, Cardiff University, Museum Avenue, Cardiff CF10 3US, UK1.
Microbiology (Reading). 2000 Sep;146 ( Pt 9):2277-2282. doi: 10.1099/00221287-146-9-2277.
Escherichia coli was adapted to grow in medium containing substantially elevated concentrations of either Zn(II), Cd(II), Co(II) or Ni(II). Whole-genome transcriptional profiles were generated from adapted strains and analysed for significant alteration in transcript abundance with reference to a wild-type strain. Similar alterations in specific message levels were observed for strains adapted to the four metal ions. One unexpected trend was the increase in transcript level of genes involved in transposition of IS elements, particularly insA. Subsequent expression of insA-7 from a heterologous promoter in E. coli conferred tolerance to Zn(II).
使大肠杆菌适应于在含有大幅提高浓度的锌（II）、镉（II）、钴（II）或镍（II）的培养基中生长。从适应后的菌株中生成全基因组转录谱，并参照野生型菌株分析转录本丰度的显著变化。对于适应四种金属离子的菌株，观察到特定信息水平有类似变化。一个意外的趋势是，参与IS元件转座的基因，特别是insA的转录水平增加。随后，在大肠杆菌中从异源启动子表达insA-7赋予了对锌（II）的耐受性。
