Comprehensive Genome Analysis on the Novel Species DCY99 Reveals Insights into Iron Tolerance of Ginseng.

Plant growth-promoting rhizobacteria play vital roles not only in plant growth, but also in reducing biotic/abiotic stress. DCY99 is isolated from soil and root of with rusty root disease, characterized by raised reddish-brown root and this is seriously affects ginseng cultivation. To investigate the relationship between 159 sequenced strains, pan-genome analysis was carried out, which suggested genomic diversity of the genus. Comparative analysis of DCY99 with sp. LK11 revealed plant growth-promoting potential of DCY99 through indole acetic acid production, phosphate solubilizing, and antifungal abilities. Detailed genomic analysis has shown that DCY99 contain various heavy metals resistance genes in its genome and the plasmid. Functional analysis with EPA505 predicted that DCY99 possess genes for degradation of polyaromatic hydrocarbon and phenolic compounds in rusty-ginseng root. Interestingly, when primed ginseng with . DCY99 during high concentration of iron exposure, iron stress of ginseng was suppressed. In order to detect DCY99 in soil, biomarker was designed using gene. This study brings new insights into the role of . DCY99 as a microbial inoculant to protect ginseng plants against rusty root disease.