College of Life Science, Kyung Hee University, Yongin 16710, Korea.
School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST), Ulsan 44919, Korea.
Int J Mol Sci. 2020 Mar 16;21(6):2019. doi: 10.3390/ijms21062019.
Plant growth-promoting rhizobacteria play vital roles not only in plant growth, but also in reducing biotic/abiotic stress. DCY99 is isolated from soil and root of with rusty root disease, characterized by raised reddish-brown root and this is seriously affects ginseng cultivation. To investigate the relationship between 159 sequenced strains, pan-genome analysis was carried out, which suggested genomic diversity of the genus. Comparative analysis of DCY99 with sp. LK11 revealed plant growth-promoting potential of DCY99 through indole acetic acid production, phosphate solubilizing, and antifungal abilities. Detailed genomic analysis has shown that DCY99 contain various heavy metals resistance genes in its genome and the plasmid. Functional analysis with EPA505 predicted that DCY99 possess genes for degradation of polyaromatic hydrocarbon and phenolic compounds in rusty-ginseng root. Interestingly, when primed ginseng with . DCY99 during high concentration of iron exposure, iron stress of ginseng was suppressed. In order to detect DCY99 in soil, biomarker was designed using gene. This study brings new insights into the role of . DCY99 as a microbial inoculant to protect ginseng plants against rusty root disease.
植物促生根际细菌不仅在植物生长中起着至关重要的作用，而且在减轻生物/非生物胁迫方面也起着重要作用。DCY99 是从锈病根的土壤和根部中分离出来的，其特征是出现凸起的红棕色根，这严重影响了人参的种植。为了研究 159 株测序菌株之间的关系，进行了全基因组分析，这表明了该属的基因组多样性。与 sp. LK11 的比较分析揭示了 DCY99 通过产生吲哚乙酸、溶解磷酸盐和抗真菌能力来促进植物生长的潜力。详细的基因组分析表明，DCY99 在其基因组和质粒中含有各种重金属抗性基因。利用 EPA505 进行功能分析预测，DCY99 拥有降解多环芳烃和酚类化合物的基因，这些化合物存在于锈病根中。有趣的是，当用人参 DCY99 对人参进行预处理，使其在高浓度铁暴露下，人参的铁胁迫得到了抑制。为了在土壤中检测 DCY99，使用基因设计了生物标志物。这项研究为人参促生菌 DCY99 作为一种微生物接种剂来保护人参植物免受锈病根病提供了新的见解。
